Evidence of actin in the cytoskeleton of microsporidia

被引：6

作者：

Bigliardi, E

Riparbelli, MG

Selmi, MG

Bini, L

Liberatori, S

Pallini, V

Bernuzzi, A

Gatti, S

Scaglia, M

Sacchi, L

机构：

[1] Univ Siena, Dept Evolutionary Biol, I-53100 Siena, Italy

[2] Univ Siena, Dept Mol Biol, I-53100 Siena, Italy

[3] IRCCS S Matteo, Parasitol Lab, Virol Serv, I-27100 Pavia, Italy

[4] Univ Pavia, IRCCS S Matteo, Infect Dis Res Lab, I-27100 Pavia, Italy

[5] Univ Pavia, Dept Anim Biol, I-27100 Pavia, Italy

来源：

JOURNAL OF EUKARYOTIC MICROBIOLOGY | 1999年 / 46卷 / 04期

关键词：

cytoskeletal proteins; Encephalitozoan cuniculi; Encephalitozoon hellem; fluorescence microscopy; immunoelectron-microscopy; two-dimensional electrophoresis;

D O I：

10.1111/j.1550-7408.1999.tb04621.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Using transmission electron microscopy, fluorescence microscopy, immuno-electron microscopy, and biochemical techniques such as 2-D electrophoresis and immunoblotting, actin was found in all biological stages of the microsporidia Encephalitozoon hellem and Encephalitozoon cuniculi.

引用

收藏

页码：410 / 415

页数：6

相关论文

共 19 条

[1] THE MELANIE PROJECT - FROM A BIOPSY TO AUTOMATIC PROTEIN MAP INTERPRETATION BY COMPUTER [J].

APPEL, RD ;

HOCHSTRASSER, DF ;

FUNK, M ;

VARGAS, JR ;

PELLEGRINI, C ;

MULLER, AF ;

SCHERRER, JR .

ELECTROPHORESIS, 1991, 12 (10) :722-735

[2] Mechanisms of microsporidial cell division:: Ultrastructural study on Encephalitozoon hellem [J].

Bigliardi, E ;

Riparbelli, MG ;

Selmi, MG ;

Lanzarini, P ;

Corona, S ;

Gatti, S ;

Scaglia, M ;

Sacchi, L .

JOURNAL OF EUKARYOTIC MICROBIOLOGY, 1998, 45 (03) :347-351

[3] THE FOCUSING POSITIONS OF POLYPEPTIDES IN IMMOBILIZED PH GRADIENTS CAN BE PREDICTED FROM THEIR AMINO-ACID-SEQUENCES [J].

BJELLQVIST, B ;

HUGHES, GJ ;

PASQUALI, C ;

PAQUET, N ;

RAVIER, F ;

SANCHEZ, JC ;

FRUTIGER, S ;

HOCHSTRASSER, D .

ELECTROPHORESIS, 1993, 14 (10) :1023-1031

[4] A NONLINEAR WIDE-RANGE IMMOBILIZED PH GRADIENT FOR 2-DIMENSIONAL ELECTROPHORESIS AND ITS DEFINITION IN A RELEVANT PH SCALE [J].

BJELLQVIST, B ;

PASQUALI, C ;

RAVIER, F ;

SANCHEZ, JC ;

HOCHSTRASSER, D .

ELECTROPHORESIS, 1993, 14 (12) :1357-1365

[5]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[6]

Canning Elizabeth U., 1993, P299

[7]

EDLIND T, 1994, J EUKARYOT MICROBIOL, V41, pS38

[8] FLUORESCENCE MICROSCOPY - REDUCED PHOTOBLEACHING OF RHODAMINE AND FLUORESCEIN PROTEIN CONJUGATES BY NORMAL-PROPYL GALLATE [J].

GILOH, H ;

SEDAT, JW .

SCIENCE, 1982, 217 (4566) :1252-1255

[9] THE CURRENT STATE OF TWO-DIMENSIONAL ELECTROPHORESIS WITH IMMOBILIZED PH GRADIENTS [J].

GORG, A ;

POSTEL, W ;

GUNTHER, S .

ELECTROPHORESIS, 1988, 9 (09) :531-546

[10] DEVELOPMENT OF POLYACRYLAMIDE GELS THAT IMPROVE THE SEPARATION OF PROTEINS AND THEIR DETECTION BY SILVER STAINING [J].

HOCHSTRASSER, DF ;

PATCHORNIK, A ;

MERRIL, CR .

ANALYTICAL BIOCHEMISTRY, 1988, 173 (02) :412-423