Identification of wheat chromosomal regions containing expressed resistance genes

The objectives of this study were to isolate and physically localize expressed resistance (R) genes oil wheat Chromosomes. Irrespective of the host or pest type, most of the 46 cloned R genes From 12 plant species share a strong sequence similarity, especially for Protein domains mid motifs. BY utilizing this perform modified RNA fingerprinting mid data mining, we identified 184 putative structural similarity to perform expressed R genes of wheat. These include 87 NB/LRR types, 16 receptor-like kinases, and 13 Pto-like kinases. The remaining were seven Hm1 and two Hs1(pro-1) homologs, 17 pathogenicity related, and 42 unique NB/kinases. About 76% of the expressed R-gene candidates were rare transcripts, including 42 novel sequences. Physical mapping of 121 candidate R-gene sequences using deletion lines localized 310 loci to 26 chromosomal regions encompassing similar to16% of die wheat genome. Five major R-gene clusters that spanned only similar to3% of the wheat genome but contained similar to47% of the candidate R genes were observed. Comparative mapping localized 91% (82 of 90) of the phenotypically characterized H genes to 18 regions where 118 of the R-gene sequences mapped.