Detection of a novel plasma serine protease during purification of vitamin K-dependent coagulation factors

被引：50

作者：

Hunfeld, A ^{[1
]}

Etscheid, M ^{[1
]}

König, H ^{[1
]}

Seitz, R ^{[1
]}

Dodt, J ^{[1
]}

机构：

[1] Paul Ehrlich Inst, D-63225 Langen, Germany

来源：

FEBS LETTERS | 1999年 / 456卷 / 02期

关键词：

plasma serine protease; vitamin K-dependent coagulation factor; plasma hyaluronan binding protease (PHBP); hepatocyte growth factor activator (HGFA)-like;

D O I：

10.1016/S0014-5793(99)00959-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel serine protease (PHBSP) was purified from human plasma by two chromatographic steps with a final yield of 1.6 mg/l plasma, The protease consists of two disulfide-bridged chains of about 50 and 30 kDa with the light chain containing the active site of the enzyme. NH2-terminal sequence analysis revealed identity to the deduced amino acid sequence of HGFA-like mRNA, The activity of PHBSP is strongly dependent on Ca2+ ions and is efficiently inhibited by alpha(2)-antiplasmin and aprotinin, Possible functions of PHBSP in the hemostatic system are discussed. (C) 1999 Federation of European Biochemical Societies.

引用

页码：290 / 294

页数：5

共 15 条

[1] EPIDERMAL GROWTH FACTOR-LIKE MODULES [J].