In vitro evaluation of biomimetic nanocomposite scaffold using endometrial stem cell derived osteoblast-like cells

被引：44

作者：

Azami, Mahmoud ^{[1
]}

Ai, Jafar ^{[1
,2
]}

Ebrahimi-Barough, Somayeh ^{[1
]}

Farokhi, Mehdi ^{[3
]}

Fard, Sahar E. ^{[4
]}

机构：

[1] Univ Tehran Med Sci, Dept Tissue Engn, Sch Adv Technol Med, Tehran, Iran

[2] Univ Tehran Med Sci, Brain & Spinal Injury Res Ctr, Tehran, Iran

[3] Baqiyatallah Univ Med Sci, Appl Biotechnol Res Ctr, Tehran, Iran

[4] Univ Tehran, Fac New Sci & Technol, Tehran, Iran

来源：

TISSUE & CELL | 2013年 / 45卷 / 05期

关键词：

Endometrial stem cells; Osteoblast cells; Biomimetic nanocomposite; Bone tissue engineeringa; AMORPHOUS CALCIUM-PHOSPHATE; BONE MORPHOGENETIC PROTEINS; MARROW STROMAL CELLS; COMPOSITE SCAFFOLDS; CHITOSAN MEMBRANE; APATITE FORMATION; DIFFUSION SYSTEM; HYDROXYAPATITE; REGENERATION; ADULT;

D O I：

10.1016/j.tice.2013.05.002

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100123 [人体微生态学]; 100210 [外科学];

摘要：

Current study encourages the differentiation of human endometrial stem cells into osteoblast-like cells using osteogenic media for potential bone tissue engineering purposes. A biomimetic nanocomposite scaffolds based on GEL/calcium phosphate were fabricated and behavior of differentiated osteoblast cells was evaluated after seeding on this scaffold. Prepared scaffolds were assessed in terms of attachment, alkaline phosphatase activity, gene expression and proliferation of osteoblast cells. The matrix mineralization was approved by alizarin red and the treated cultures with osteogenic media and BMP2 were positive for osteopontin and osteocalcin antibodies. RT-PCR confirmed presence of osteopontin, osteonectin and alkaline phosphatase mRNA after differentiation in EnSCs-derived osteoblast-like cells. Also, it has been shown that the biomimetic nanocomposites possess appropriate chemical and physical properties to support the attachment and proliferation of differentiated osteoblast cells. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：328 / 337

页数：10

共 58 条

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Preparation, characterization and mechanical properties of controlled porous gelatin/hydroxyapatite nanocomposite through layer solvent casting combined with freeze-drying and lamination techniques [J].