SHN-1, a Shank homologue in C-elegans, affects defecation rhythm via the inositol-1,4,5-trisphosphate receptor

被引:16
作者
Jee, C
Lee, J
Lee, JI
Lee, WH
Park, BJ
Yu, JR
Park, E
Kim, E
Ahnn, J [1 ]
机构
[1] Kwangju Inst Sci & Technol, Dept Life Sci, Kwangju 500712, South Korea
[2] Konkuk Univ, Coll Med, Dept Parasitol, Chungju 380710, South Korea
[3] Korea Adv Inst Sci & Technol, Creat Res Ctr Synaptogenesis, Taejon 305701, South Korea
[4] Korea Adv Inst Sci & Technol, Dept Biol Sci, Taejon 305701, South Korea
关键词
inositol-1,4,5-trisphosphate receptor; postsynaptic density; scaffolding protein; receptor localization; defecation cycle;
D O I
10.1016/S0014-5793(04)00107-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Protein localization in the postsynaptic density (PSD) of neurons is mediated by scaffolding proteins such as PSD-95 and Shank, which ensure proper function of receptors at the membrane. The Shank family of scaffolding proteins contain PDZ (PSD-95, Dig, and ZO-1) domains and have been implicated in the localizations of many receptor proteins including glutamate receptors in mammals. We have identified and characterized shn-1, the only homologue of Shank in Caenorhahditis elegans. The shn-1 gene shows approximately 40% identity over 1000 amino acids to rat Shanks. SHN-1 protein is localized in various tissues including neurons, pharynx and intestine. RNAi suppression of SHN-1 did not cause lethality or developmental abnormality. However, suppression of SHN-1 in the itr-1 (sa73) mutant, which has a defective inositol-1,4,5-trisphosphate (IP3) receptor, resulted in animals with altered defecation rhythm. Our data suggest a possible role of SHN-1 in affecting function of IP3 receptors in C. elegans. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码:29 / 36
页数:8
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