Cloning of the gene encoding honeybee long-wavelength rhodopsin: A new class of insect visual pigments

被引：41

作者：

Chang, BSW

Ayers, D

Smith, WC

Pierce, NE

机构：

[1] HARVARD UNIV, MUSEUM COMPARAT ZOOL LABS, DEPT ORGANISM & EVOLUT BIOL, CAMBRIDGE, MA 02138 USA

[2] UNIV FLORIDA, DEPT OPHTHALMOL, GAINESVILLE, FL 32610 USA

来源：

GENE | 1996年 / 173卷 / 02期

关键词：

molecular evolution; vision; Hymenoptera; G protein-coupled receptor; Apis mellifera; phylogeny; wavelength regulation;

D O I：

10.1016/0378-1119(96)00165-5

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Rhodopsins (Rh), G-protein-coupled receptors with seven transmembrane (TM) helices, form the first step in visual transduction in most organisms. Although many long-wavelength (LW) vertebrate opsin sequences are known, less information is available for invertebrate LW sequences. By a combination of RT-PCR and cDNA library screening, we have cloned and sequenced the honeybee LW Rh gene. The deduced protein is composed of 378 amino acids (aa), appears to have seven TM regions, and contains many of the structures and key aa thought to be important for Rh function. Phylogenetic analysis of this sequence in relation to other invertebrate Rh reveals it to be a member of a new group of insect LW Rh.

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页码：215 / 219

页数：5

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