Characterization of photosystem II activity and heterogeneity during the cell cycle of the green alga Scenedesmus quadricauda

被引:41
作者
Kaftan, D
Meszaros, T
Whitmarsh, J
Nedbal, L
机构
[1] Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA
[2] Inst Microbiol, NRC Photosynth & Global Climate Change, CZ-37981 Trebon, Czech Republic
[3] S Bohemian Univ, CZ-37005 Ceske Budejovice, Czech Republic
[4] ARS, Photosynth Res Unit, USDA, Urbana, IL 61801 USA
关键词
D O I
10.1104/pp.120.2.433
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The photosynthetic activity of the green alga Scenedesmus quadricauda was investigated during synchronous growth in light/dark cycles. The rate of O-2 evolution increased 2-fold during the first 3 to 4 h of the light period, remained high for the next 3 to 4 h, and then declined during the last half of the light period. During cell division, which occurred at the beginning of the dark period, the ability of the cells to evolve O-2 was at a minimum. To determine ii photosystem II (PSII) controls the photosynthetic capacity of the cells during the cell cycle we measured PSII activity and heterogeneity. Measurements of electron-transport activity revealed two populations of PSII, active centers that contribute to carbon reduction and inactive centers that do not. Measurements of PSII antenna sizes also revealed two populations, PSIIalpha and PSIIbeta, which differ from one another by their antenna size. During the early light period the photosynthetic capacity of the cells doubled, the O-2-evolving capacity of PSII was nearly constant, the proportion of PSIIbeta centers decreased to nearly zero, and the proportion of inactive PSII centers remained constant. During the period of minimum photosynthetic activity 30% of the PSII centers were insensitive to the inhibitor 3-(3,4-dichlorophenyl)-1,1-dimethylurea, which may be related to reorganization of the thylakoid membrane. We conclude from these results that PSII does not limit the photosynthetic activity of the cells during the first half of the light period. However, the decline in photosynthetic activity observed during the last half of the light period can be accounted for by limited PSII activity.
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页码:433 / 441
页数:9
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