Manipulation of single coiled DNA molecules by laser clustering of microparticles

被引：41

作者：

Hirano, K

Baba, Y

Matsuzawa, Y

Mizuno, A

机构：

[1] Univ Tokushima, Fac Pharmaceut Sci, Dept Med Chem, Tokushima 7708505, Japan

[2] Japan Sci & Technol Corp, CREST, Tokushima 7708505, Japan

[3] Toyohashi Univ Technol, Dept Ecol Engn, Aichi 4418580, Japan

来源：

APPLIED PHYSICS LETTERS | 2002年 / 80卷 / 03期

关键词：

D O I：

10.1063/1.1435803

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

We have developed a method of manipulating single DNA molecules for application in single-molecule analysis. Using a bead cluster formed by laser trapping, the technique allows single DNA molecules to be manipulated at any point on the molecule without the need for prior chemical modification as in DNA-bead complex techniques. We describe the method and the characteristics of cluster formation, and present examples of actual DNA molecule manipulation. (C) 2002 American Institute of Physics.

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页码：515 / 517

页数：3

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