Influence of the alternative σ28 factor on virulence and flagellum expression of Legionella pneumophila

被引：55

作者：

Heuner, K ^{[1
]}

Dietrich, C ^{[1
]}

Skriwan, C ^{[1
]}

Steinert, M ^{[1
]}

Hacker, J ^{[1
]}

机构：

[1] Univ Wurzburg, Inst Molekulare Infektiosbiol, D-97070 Wurzburg, Germany

来源：

INFECTION AND IMMUNITY | 2002年 / 70卷 / 03期

关键词：

D O I：

10.1128/IAI.70.3.1604-1608.2002

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The fliA gene of Legionella pneumophila encoding the alternative sigma(28) factor was inactivated by introducing a kanamycin resistance cassette. Electron microscopy and Western blot analysis revealed that the fliA mutant strain is aflagellate and expresses no flagellin. Reporter gene assays indicated that the flaA promoter is not active in the fliA mutant strain. The fliA mutant strain multiplied less effectively in coculture with amoebae than the wild-type strain and was not able to replicate in coculture with Dictyostelium discoideum.

引用

页码：1604 / 1608

页数：5

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