yellow and ebony Are the Responsible Genes for the Larval Color Mutants of the Silkworm Bombyx Mori

被引:139
作者
Futahashi, Ryo [1 ,2 ]
Sato, Jotaro [3 ]
Meng, Yan [3 ]
Okamoto, Shun [1 ]
Daimon, Takaaki [3 ]
Yamamoto, Kimiko [2 ]
Suetsugu, Yoshitaka [2 ]
Narukawa, Junko [2 ]
Takahashi, Hirokazu [2 ]
Banno, Yutaka [4 ]
Katsuma, Susumu [3 ]
Shimada, Toru [3 ]
Mita, Kazuei [2 ]
Fujiwara, Haruhiko [1 ]
机构
[1] Univ Tokyo, Grad Sch Frontier Sci, Dept Integrat Biosci, Chiba 2778562, Japan
[2] Natl Inst Agrobiol Sci, Lab Insect Genome, Tsukuba, Ibaraki 3058643, Japan
[3] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Agr & Environm Biol, Bunkyo Ku, Tokyo 1138657, Japan
[4] Kyushu Univ, Fac Agr, Lab Insect Genet Resources, Fukuoka 8128581, Japan
关键词
D O I
10.1534/genetics.108.096388
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Many larval color mutants; have been obtained in the silkworm Bombyx mori. Mapping of melaninsynthesis genes oil the Bombyx linkage map revealed that yellow and ebony genes were located near the chocolate (ch) and sooly (so) loci, respectively. In the ch mutants, body color of neonate larvae and the body markings of elder instar larvae are reddish brown instead of normal black. Mutations at the so locus produce smoky larvae and black pupae. F-2 linkage analyses showed that. sequence polymorphisms of yellow and ebony genes perfectly cosegregated with the ch and so mutant phenotypes, respectively. Both yellow and ebony were expressed in the epidermis during the molting period when cuticular pigmentation occurred. The spatial expression pattern of yellow transcripts coincided with the larval black markings. In the ch mutants, nonsense mutations of the yellow gene were detected, whereas large deletions of the ebony ORF were detected in the so mutants. These results indicate that yellow and ebony are the responsible genes for the ch and so loci, respectively. Our findings suggest that Yellow promotes melanization, whereas Ebony inhibits melanization in Lepidoptera and that melanin-synthesis enzymes play a critical role in the lepiclopteran larval color pattern.
引用
收藏
页码:1995 / 2005
页数:11
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