An improved method for the synthesis of N3'->P5' phosphoramidate oligonucleotides

被引：24

作者：

McCurdy, SN ^{[1
]}

Nelson, JS ^{[1
]}

Hirschbein, BL ^{[1
]}

Fearon, KL ^{[1
]}

机构：

[1] LYNX THERAPEUT,HAYWARD,CA 94545

来源：

TETRAHEDRON LETTERS | 1997年 / 38卷 / 02期

关键词：

D O I：

10.1016/S0040-4039(96)02254-X

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A new method for the synthesis of N3'-->P5' phosphoramidate oligonucleotides is demonstrated. This phosphoramidite amine-exchange method generates cycle yields in the 92-95% range, and provides overall yields that are 3-6 times higher than the previously published oxidative phosphorylation method. Copyright (C) 1996 Elsevier Science Ltd

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收藏

页码：207 / 210

页数：4

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