Protective effect of panaxydol and panaxynol on sodium nitroprusside-induced apoptosis in cortical neurons

被引:33
作者
Nie, BM
Yang, LM
Fu, SL
Jiang, XY
Lu, PH
Lu, Y
机构
[1] Shanghai Jiao Tong Univ, Sch Med, Pharmaceut Res Inst, Shanghai 200025, Peoples R China
[2] Shanghai Jiao Tong Univ, Sch Med, Dept Neurobiol, Shanghai 200025, Peoples R China
基金
中国国家自然科学基金;
关键词
sodium nitroprusside; cortical neurons; Alzheimer's disease; apoptosis; panaxydol; panaxynol;
D O I
10.1016/j.cbi.2006.02.001
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
An excess of the free radical nitric oxide (NO) is viewed as a deleterious factor involved in various CNS disorders. The protective effect of panaxydol (PND) and panaxynol (PNN) on sodium nitroprusside (SNP)-induced neuronal apoptosis and potential mechanism were investigated in primary cultured rat cortical neurons. Pretreatment of the cells with PND or PNN for 24 h following 1 mM SNP, an exogenous NO donor, exposure for I h, resulted significantly in reduction of cell death induced by SNP determined by MTT assay, LDH release and Hoechst staining. 5 mu M PND and PNN also reduced the up-regulation of the pro-apoptotic gene, Bax, down-regulation of the anti-apoptotic gene, Bcl-2. The observations demonstrated that PND and PNN protect neurons against SNP-induced apoptosis via regulating the apoptotic related genes. The results raise the possibility that PND and PNN reduce neurodegeneration in the Alzheimer's brain. (c) 2006 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:225 / 231
页数:7
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