CTCF, a candidate trans-acting factor for X-inactivation choice

被引：219

作者：

Chao, W ^{[1
]}

Huynh, KD ^{[1
]}

Spencer, RJ ^{[1
]}

Davidow, LS ^{[1
]}

Lee, JT ^{[1
]}

机构：

[1] Harvard Univ, Massachusetts Gen Hosp, Sch Med,Dept Genet, Howard Hughes Med Inst,Dept Mol Biol, Boston, MA 02114 USA

来源：

SCIENCE | 2002年 / 295卷 / 5553期

关键词：

D O I：

10.1126/science.1065982

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In mammals, X-inactivation silences one of two female X chromosomes. Silencing depends on the noncoding gene, Xist ((i) under bar nactive (X) under bar-(s) under bar pecific (t) under bar ranscript), and is blocked by the antisense gene, Tsix. Deleting the choice/imprinting center in Tsix affects X-chromosome selection. Here, we identify the insulator and transcription factor, CTCF, as a candidate trans-acting factor for X-chromosome selection. The choice/imprinting center contains tandem CTCF binding sites that function in an enhancer-blocking assay. In vitro binding is reduced by CpG methylation and abolished by including non-CpG methylation. We postulate that Tsix and CTCF together establish a regulatable epigenetic switch for X-inactivation.

引用

页码：345 / 347

页数：3

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