Structure and RNA-binding properties of the Not1-Not2Not5 module of the yeast Ccr4-Not complex

被引：61

作者：

Bhaskar, Varun ^{[1
]}

Roudko, Vladimir ^{[2
,3
]}

Basquin, Jerome ^{[1
]}

Sharma, Kundan ^{[4
]}

Urlaub, Henning ^{[4
]}

Seraphin, Bertrand ^{[2
,3
]}

Conti, Elena ^{[1
]}

机构：

[1] Max Planck Inst Biochem, Dept Struct Cell Biol, D-82152 Martinsried, Germany

[2] CNRS, IGBMC, INSERM, UMR 7104,U964, Illkirch Graffenstaden, France

[3] Univ Strasbourg, Illkirch Graffenstaden, France

[4] Max Planck Inst Biophys Chem, D-37077 Gottingen, Germany

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2013年 / 20卷 / 11期

基金：

欧洲研究理事会;

关键词：

OPEN-SOURCE SOFTWARE; MESSENGER-RNAS; TRANSCRIPTION FACTOR; DEADENYLASE COMPLEX; GENE-EXPRESSION; CAF1; PROTEINS; CORE DOMAIN; REGULATOR; RECRUITMENT; COMPONENTS;

D O I：

10.1038/nsmb.2686

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

The Ccr4-Not complex is involved in several aspects of gene expression, including mRNA decay, translational repression and transcription. We determined the 2.8-angstrom-resolution crystal structure of a 120-kDa core complex of the Saccharomyces cerevisiae Not module comprising the C-terminal arm of Not1, Not2 and Not5. Not1 is a HEAT-repeat scaffold. Not2 and Not5 have extended regions that wrap around Not1 and around their globular domains, the Not boxes. The Not boxes resemble Sm folds and interact with each other with a noncanonical dimerization surface. Disruption of the interactions within the ternary complex has severe effects on growth in vivo. The ternary complex forms a composite surface that binds poly( U) RNA in vitro, with a site at the Not5 Not box. The results suggest that the Not module forms a versatile platform for macromolecular interactions.

引用

页码：1281 / U207

页数：10

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