Neurogenesis in the central olfactory pathway of the adult shore crab Carcinus maenas is controlled by sensory afferents

被引:32
作者
Hansen, A
Schmidt, M
机构
[1] Univ Hamburg, Inst Zool, D-20146 Hamburg, Germany
[2] Univ Hamburg, Zool Museum, D-20146 Hamburg, Germany
关键词
olfaction; crustaceans; cell proliferation; progeny; BrdU;
D O I
10.1002/cne.1408
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The number of olfactory projection neurons (OPNs) in the brain of the juvenile and adult shore crab Carcinus maenas continues to increase during the life of the animal. In vivo labeling of adult crabs with the proliferation marker bromodeoxyuridine (BrdU) revealed a group of proliferating neuronal precursor cells in the lateral soma clusters (LCs) and in the soma clusters of the hemiellipsoid bodies (HBCs). The LCs contain the cell bodies of the olfactory projection neurons and the HBCs house the cell bodies to which the OPNs project. The aim of the present study was to examine whether the input from primary olfactory afferents has any influence on the rate of proliferation and survival of the neuronal precursors in the central olfactory pathways of C. maenas. Different sets of experiments involving BrdU injection and its immunocytochemical detection combined with unilateral amputation of the antennule that houses the olfactory organ were carried out. Our results show that the missing olfactory sensory input affects the rate of proliferation and the survival of postmitotic cells in the LC and in the HBC compared with control animals. The effect on the survival of postmitotic cells tested by BrdU injection followed by unilateral ablation is lateralized. Proliferation of neuronal precursor cells tested by the reversed experimental order was drastically impaired bilaterally. We conclude that the olfactory sensory input is necessary for a normal rate of proliferation of neuronal precursors and the survival of their progeny in the LC and in the HBC of C. maenas. (C) 2001 Wiley-Liss, Inc.
引用
收藏
页码:223 / 233
页数:11
相关论文
共 47 条
[1]   AUTORADIOGRAPHIC AND HISTOLOGICAL STUDIES OF POSTNATAL NEUROGENESIS .4. CELL PROLIFERATION AND MIGRATION IN ANTERIOR FOREBRAIN, WITH SPECIAL REFERENCE TO PERSISTING NEUROGENESIS IN OLFACTORY BULB [J].
ALTMAN, J .
JOURNAL OF COMPARATIVE NEUROLOGY, 1969, 137 (04) :433-&
[2]   TRACER DOSE AND AVAILABILITY TIME OF THYMIDINE AND BROMODEOXYURIDINE - APPLICATION OF BROMODEOXYURIDINE IN CELL KINETIC-STUDIES [J].
BOSWALD, M ;
HARASIM, S ;
MAURERSCHULTZE, B .
CELL AND TISSUE KINETICS, 1990, 23 (03) :169-181
[3]   UNILATERAL ODOR DEPRIVATION - TIME COURSE OF CHANGES IN LAMINAR VOLUME [J].
BRUNJES, PC .
BRAIN RESEARCH BULLETIN, 1985, 14 (03) :233-237
[4]  
BRUNJES PC, 1994, BRAIN RES REV, V19, P146
[5]  
BURD GD, 2000, NEUROBIOLOGY TASTE S, P233
[6]  
Cummings DM, 1997, J NEUROBIOL, V33, P429, DOI 10.1002/(SICI)1097-4695(199710)33:4<429::AID-NEU7>3.0.CO
[7]  
2-C
[8]  
Farbman A.I., 1992, CELL BIOL OLFACTION
[9]   UNILATERAL ODOR DEPRIVATION - EARLY POSTNATAL CHANGES IN OLFACTORY-BULB CELL-DENSITY AND NUMBER [J].
FRAZIER, LL ;
BRUNJES, PC .
JOURNAL OF COMPARATIVE NEUROLOGY, 1988, 269 (03) :355-370
[10]   DEVELOPMENTAL-STUDY OF AFFERENTED AND DEAFFERENTED BEE ANTENNAL LOBES [J].
GASCUEL, J ;
MASSON, C .
JOURNAL OF NEUROBIOLOGY, 1991, 22 (08) :795-810