DNA-binding by functionalized gold nanoparticles: Mechanism and structural requirements

被引：68

作者：

Goodman, CM ^{[1
]}

Chari, NS ^{[1
]}

Han, G ^{[1
]}

Hong, R ^{[1
]}

Ghosh, P ^{[1
]}

Rotello, VM ^{[1
]}

机构：

[1] Univ Massachusetts, Dept Chem, Amherst, MA 01003 USA

来源：

CHEMICAL BIOLOGY & DRUG DESIGN | 2006年 / 67卷 / 04期

关键词：

DNA; intercalator; hydrophobicity; LC50; nanoparticle;

D O I：

10.1111/j.1747-0285.2006.00372.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A family of nanoparticles featuring surfaces of varying hydrophobicity was synthesized. The efficiency of DNA-binding was determined, demonstrating in a fivefold modulation in binding a 37-mer DNA strand. Nanoparticle-binding causes a reversible conformational change in the DNA structure, as demonstrated by circular dichroism and fluorescence experiments. Furthermore, the affinity of the nanoparticle for the DNA can be regulated by external agents, though stability of the complex is observed at relatively high ionic strengths.

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收藏

页码：297 / 304

页数：8

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