A Zn(II)-translocating P-type ATPase from Proteus mirabilis

被引：23

作者：

Rensing, C ^{[1
]}

Mitra, B ^{[1
]}

Rosen, BP ^{[1
]}

机构：

[1] Wayne State Univ, Sch Med, Dept Biochem & Mol Biol, Detroit, MI 48201 USA

来源：

BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE | 1998年 / 76卷 / 05期

关键词：

zinc resistance; P-type ATPase; Proteus mirabilis;

D O I：

10.1139/bcb-76-5-787

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A mutant of Proteus mirabilis had been previously isolated as defective in swarming. The mutation had been found to be in a gene related to the Escherichia coli zntA gene, which encodes the ZntA Zn(II)-translocating P-type ATPase. In this study the P. mirabilis gene was expressed in an E. coli strain in which the zntA gene had been disrupted. The P. mirabilis gene complemented the sensitivity to salts of zinc and cadmium. Everted membrane vesicles from the zntA-disrupted strain lost ATP-driven Zn-65(II) uptake. Membranes from the complemented strain had restored Zn-65(II) transport. These results demonstrate that the P. mirabilis homologue of ZntA is a Zn(II)-translocating P-type ATPase.

引用

页码：787 / 790

页数：4

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