A toxin to nervous, cardiac, and endocrine ERG K+ channels isolated from Centruroides noxius scorpion venom

被引：92

作者：

Gurrola, GB

Rosati, B

Rocchetti, M

Pimienta, G

Zaza, A

Arcangeli, A

Olivotto, M

Possani, LD

Wanke, E

机构：

[1] Univ Milan, Dipartimento Fisiol & Biochim Gen, I-20133 Milan, Italy

[2] Univ Milano Bicocca, Dept Biotechnol & Biosci, I-20126 Milan, Italy

[3] Univ Milan, Dept Gen Physiol & Biochem, Lab Electrophysiol, I-20133 Milan, Italy

[4] Natl Autonomous Univ Mexico, Inst Biotechnol, Dept Mol Recognit & Struct Biol, Cuernavaca 62271, Morelos, Mexico

[5] Univ Florence, Inst Gen Pathol, I-50134 Florence, Italy

来源：

FASEB JOURNAL | 1999年 / 13卷 / 08期

关键词：

eag gene family; erg gene; ergtoxin; scorpion toxin; action potential;

D O I：

10.1096/fasebj.13.8.953

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Toxins isolated from a variety of venoms are tools for probing the physiological function and structure of ion channels. The ether-a-go-go-related genes (erg) codify for the K+ channels (ERG), which are crucial in neurons and are impaired in human long-QT syndrome and Drosophila 'seizure' mutants. We have isolated a peptide from the scorpion Centruroides noxius Hoffmann that has no sequence homologies with other toxins, and demonstrate that it specifically inhibits (IC50=16+/-1 nM) only ERG channels of different species and distinct histogenesis. These results open up the possibility of investigating ERG channel structure-function relationships and novel pharmacological tools with potential therapeutic efficacy.

引用

页码：953 / 962

页数：10

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