Estimation of growth rates of Escherichia coli BJ4 in streptomycin-treated and previously germfree mice by in situ rRNA hybridization

被引:41
作者
Rang, CU
Licht, TR
Midtvedt, T
Conway, PL
Chao, L
Krogfelt, KA
Cohen, PS
Molin, S
机构
[1] Tech Univ Denmark, Dept Microbiol, DK-2800 Lyngby, Denmark
[2] Univ Gothenburg, Dept Gen & Marine Microbiol, S-41319 Gothenburg, Sweden
[3] Karolinska Inst, Dept Med Microbial Ecol, S-10401 Stockholm, Sweden
[4] Statens Serum Inst, Dept Gastrointestinal Infect, DK-2300 Copenhagen, Denmark
[5] Univ New S Wales, Sch Microbiol & Immunol, Kensington, NSW 2033, Australia
[6] Univ Maryland, Dept Zool, College Pk, MD 20742 USA
[7] Univ Rhode Isl, Dept Mol Genet Biochem & Microbiol, Kingston, RI 02881 USA
关键词
D O I
10.1128/CDLI.6.3.434-436.1999
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The growth physiologv of Escherichia coli during colonization of the intestinal tract was studied with four animal models: the streptomycin-treated mouse carrying a reduced microflora, the monoassociated mouse with no other microflora than the introduced strain, the conventionalized streptomycin-treated mouse, and the conventionalized monoassociated mouse harboring a full microflora. A 23S rRNA fluorescent oligonucleotide probe was used for hybridization to whole E. coli cells fixed directly after being taken from the animals, and the respective growth rates of E. coli BJ4 in the four animal models were estimated by correlating the cellular concentrations of ribosomes with the growth rate of the strain. The growth rates thus estimated from the ribosomal content off. coli BJ4 in vivo did not differ in the streptomycin-treated and the monoassociated mice. After conventionalization there was a slight decrease of the bacterial growth rates in both animal models.
引用
收藏
页码:434 / 436
页数:3
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