Rapid isolation and identification of minor natural products by LC-MS, LC-SPE-NMR and ECD: Isoflavanones, biflavanones and bisdihydrocoumarins from Ormocarpum kirkii

被引：23

作者：

Xu, Yong-Jiang ^{[1
]}

Foubert, Kenn ^{[1
]}

Dhooghe, Liene ^{[1
]}

Lemiere, Filip ^{[2
]}

Maregesi, Sheila ^{[3
]}

Coleman, Christina M. ^{[4
,5
]}

Zou, Yike ^{[4
,5
]}

Ferreira, Daneel ^{[4
,5
]}

Apers, Sandra ^{[1
]}

Pieters, Luc ^{[1
]}

机构：

[1] Univ Antwerp, Dept Pharmaceut Sci, Lab Pharmacognosy & Pharmaceut Anal, B-2610 Antwerp, Belgium

[2] Univ Antwerp, Dept Chem, Nucleoside Res & Mass Spectrometry Unit, B-2610 Antwerp, Belgium

[3] Univ Antwerp, Dept Chem, Nucleoside Res & Mass Spectrometry Unit, B-2610 Antwerp, Belgium

[4] Univ Mississippi, Sch Pharm, Dept Pharmacognosy, University, MS 38677 USA

[5] Univ Mississippi, Sch Pharm, Pharmaceut Sci Res Inst, University, MS 38677 USA

来源：

PHYTOCHEMISTRY | 2012年 / 79卷

关键词：

Ormocarpum kirkii; Papilionaceae; LC-SPE-NMR; Antiplasmodial; Isoflavonoids; Biflavonoids; Neoflavanoids; Electronic circular dichroism (ECD); ELECTRONIC CIRCULAR-DICHROISM; ABSOLUTE-CONFIGURATION; THEORETICAL CALCULATION; PLANTS; HYPHENATION; TANZANIA; TOOL;

D O I：

10.1016/j.phytochem.2012.04.004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The combination of the hyphenated techniques LC-MS and LC-SPE-NMR constitutes a powerful platform for the rapid isolation and identification of minor components from natural sources. Electronic circular dichroism (ECD) is a useful tool to determine the absolute configuration of small quantities of chiral molecules. In order to search for minor constituents present in an Ormocarpum kirkii extract, these techniques were applied for the separation and structure elucidation of a series of isoflavanones, biflavanones and biscoumarins. After optimization of chromatographic conditions and subsequent isolation, MS and 1D and 2D NMR data were collected. Experimental and calculated ECD spectra were used in conjunction with NMR data to confirm the absolute configuration of these compounds. Eight compounds were identified for the first time and six have been previously reported. The present approach offers a strategy for accelerating research on natural products. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：121 / 128

页数：8

共 24 条

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