The calcium-activated nonselective cation channel TRPM4 is essential for the migration but not the maturation of dendritic cells

被引：170

作者：

Barbet, Gaetan ^{[1
,2
,3
]}

Demion, Marie ^{[1
,2
,3
]}

Moura, Ivan C. ^{[1
,3
]}

Serafini, Nicolas ^{[1
,2
,3
]}

Leger, Thibaut ^{[1
,2
,3
]}

Vrtovsnik, Francois ^{[1
,3
,4
]}

Monteiro, Renato C. ^{[1
,3
]}

Guinamard, Romain ^{[5
]}

Kinet, Jean-Pierre ^{[6
,7
]}

Launay, Pierre ^{[1
,2
,3
]}

机构：

[1] Univ Paris 07, INSERM, U699, F-75018 Paris, France

[2] Univ Paris 07, INSERM, Equipe Avenir, F-75018 Paris, France

[3] Univ Paris 07, Fac Med, F-75018 Paris, France

[4] Hop Bichat Claude Bernard, Serv Nephrol, F-75018 Paris, France

[5] Univ Caen, Lab Physiol Cellulaire, EA 3212, F-14032 Caen, France

[6] Harvard Univ, Sch Med, Boston, MA 02215 USA

[7] Beth Israel Deaconess Med Ctr, Dept Pathol, Boston, MA 02215 USA

来源：

NATURE IMMUNOLOGY | 2008年 / 9卷 / 10期

关键词：

D O I：

10.1038/ni.1648

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Dendritic cell (DC) maturation and migration are events critical for the initiation of immune responses. After encountering pathogens, DCs upregulate the expression of costimulatory molecules and subsequently migrate to secondary lymphoid organs. Calcium (Ca2+) entry governs the functions of many hematopoietic cell types, but the role of Ca2+ entry in DC biology remains unclear. Here we report that the Ca2+-activated nonselective cation channel TRPM4 was expressed in and controlled the Ca2+ homeostasis of mouse DCs. The absence of TRPM4, which elicited Ca2+ overload, did not influence DC maturation but did considerably impair chemokine-dependent DC migration. Our results establish TRPM4-regulated Ca2+ homeostasis as crucial for DC mobility but not maturation and emphasize that DC maturation and migration are independently regulated.

引用

页码：1148 / 1156

页数：9

共 43 条

[1] Dendritic cells as therapeutic vaccines against cancer [J].

Banchereau, J ;

Palucka, AK .

NATURE REVIEWS IMMUNOLOGY, 2005, 5 (04) :296-306

[2] Calcium oscillations regulate thymocyte motility during positive selection in the three-dimensional thymic environment [J].

Bhakta, NR ;

Oh, DY ;

Lewis, RS .

NATURE IMMUNOLOGY, 2005, 6 (02) :143-151

[3] Generation, migration and function of circulating dendritic cells [J].

Bonasio, Roberto ;

von Andrian, Ulrich H. .

CURRENT OPINION IN IMMUNOLOGY, 2006, 18 (04) :503-511

[4] SnapShot; Mammalian TRP channels [J].

Clapham, David E. .

CELL, 2007, 129 (01) :220-220

[5] CD16 promotes Escherichia coli sepsis through an FcRγ inhibitory pathway that prevents phagocytosis and facilitates inflammation [J].

da Silva, Fabiano Pinheiro ;

Aloulou, Meryem ;

Skurnik, David ;

Benhamou, Marc ;

Andremont, Antoine ;

Velasco, Irineu T. ;

Chiamolera, Murilo ;

Verbeek, J. Sjef ;

Launay, Pierre ;

Monteiro, Renato C. .

NATURE MEDICINE, 2007, 13 (11) :1368-1374

[6] Bruton's tyrosine kinase and phospholipase Cγ2 mediate chemokine-controlled B cell migration and homing [J].

de Gorter, David J. J. ;

Beuling, Esther A. ;

Kersseboom, Rogier ;

Middendorp, Sabine ;

van Gils, Janine M. ;

Hendriks, Rudolf W. ;

Pals, Steven T. ;

Spaargaren, Marcel .

IMMUNITY, 2007, 26 (01) :93-104

[7] Regulation of dendritic cell migration and adaptive immune response by leukotriene B4 receptors:: a role for LTB4 in up-regulation of CCR7 expression and function [J].

Del Prete, Annalisa ;

Shao, Wen-Hai ;

Mitola, Stefania ;

Santoro, Giuseppe ;

Sozzani, Silvano ;

Haribabu, Bodduluri .

BLOOD, 2007, 109 (02) :626-631

[8] TRPM4, a Ca2+-activated nonselective cation channel in mouse sino-atrial node cells [J].

Demion, Marie ;

Bois, Patrick ;

Launay, Pierre ;

Guinamard, Romain .

CARDIOVASCULAR RESEARCH, 2007, 73 (03) :531-538

[9] THE INTRACELLULAR CA2+ CONCENTRATION OPTIMAL FOR T-CELL ACTIVATION IS QUITE DIFFERENT AFTER IONOMYCIN OR CD3 STIMULATION [J].

DONNADIEU, E ;

BISMUTH, G ;

TRAUTMANN, A .

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1995, 429 (04) :546-554

[10] Calcium signalling in lymphocyte activation and disease [J].

Feske, Stefan .

NATURE REVIEWS IMMUNOLOGY, 2007, 7 (09) :690-702

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