Effects of hyperbaric oxygen on procollagen messenger RNA levels and collagen synthesis in the healing of rat tendon laceration

被引：50

作者：

Ishii, Y

Miyanaga, Y

Shimojo, H

Ushida, T

Tateishi, T

机构：

[1] Univ Tsukuba, Inst Hlth & Sport Sci, Tsukuba, Ibaraki 305, Japan

[2] Natl Inst Adv Interdisciplinary Res, 3D Tissue Engn Grp, Tsukuba, Ibaraki, Japan

来源：

TISSUE ENGINEERING | 1999年 / 5卷 / 03期

关键词：

D O I：

10.1089/ten.1999.5.279

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

In the present study, we examined the effect of intermittent exposure to hyperbaric oxygen (HBO) on experimentally induced tendon laceration in the right hind limb of 56 male Wistar rats. We compared animals treated by HBO (2 atmospheres absolute [ATA] 100% O-2 x 60 min x once per day) with control animals (without HBO) at 3, 7, 14, and 28 days after wound creation. Histology, and pro-alpha 1 (I) mRNA by Northern hybridization, pro-alpha 1 (III) mRNA, transforming growth factor-beta 1 (TGF-beta 1) mRNA by RT-PCR were studied. Our results indicate the use of HBO influences the pro-alpha 1 (I) mRNA expression. The level of pro-alpha 1 (I) mRNA was especially increased at 7-14 days after laceration by HBO. These results suggest that intermittent oxygen exposure may play a significant role in collagen synthesis and be beneficial for producing the extracellular matrices in tissue engineering.

引用

页码：279 / 286

页数：8

共 26 条

[1]

CONSTRUCTION OF DNA-SEQUENCES COMPLEMENTARY TO RAT ALPHA-1 AND ALPHA-2 COLLAGEN MESSENGER-RNA AND THEIR USE IN STUDYING THE REGULATION OF TYPE-I COLLAGEN-SYNTHESIS BY 1,25-DIHYDROXYVITAMIN-D [J].