A pressure system for the microinjection of substances into the brain of awake monkeys

被引：11

作者：

Dias, EC ^{[1
]}

Segraves, MA ^{[1
]}

机构：

[1] NORTHWESTERN UNIV,DEPT NEUROBIOL & PHYSIOL,EVANSTON,IL 60208

来源：

JOURNAL OF NEUROSCIENCE METHODS | 1997年 / 72卷 / 01期

关键词：

microinjection; neurophysiology; awake behaving primate;

D O I：

10.1016/S0165-0270(96)00154-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a method for placing pressure microinjections of drugs or anatomical tracers in physiologically defined sites in the brain of awake monkeys. This method provides a means to record neuronal activity from the tip of an injection cannula so that an injection can be made at a physiologically defined location. It uses pressure in a closed system to precisely control the amount of fluid injected and provides a visible means for monitoring injection volume. The injection cannula is easy to make with readily available components and can be used repeatedly for multiple recording sessions and injections. (C) 1997 Elsevier Science B.V.

引用

页码：43 / 47

页数：5