Genome sequence and analysis of the oral bacterium Fusobacterium nucleatum strain ATCC 25586

被引：279

作者：

Kapatral, V ^{[1
]}

Anderson, I ^{[1
]}

Ivanova, N ^{[1
]}

Reznik, G ^{[1
]}

Los, T ^{[1
]}

Lykidis, A ^{[1
]}

Bhattacharyya, A ^{[1
]}

Bartman, A ^{[1
]}

Gardner, W ^{[1
]}

Grechkin, G ^{[1
]}

Zhu, LH ^{[1
]}

Vasieva, O ^{[1
]}

Chu, L ^{[1
]}

Kogan, Y ^{[1
]}

Chaga, O ^{[1
]}

Goltsman, E ^{[1
]}

Bernal, A ^{[1
]}

Larsen, N ^{[1
]}

D'Souza, M ^{[1
]}

Walunas, T ^{[1
]}

Pusch, G ^{[1
]}

Haselkorn, R ^{[1
]}

Fonstein, M ^{[1
]}

Kyrpides, N ^{[1
]}

Overbeek, R ^{[1
]}

机构：

[1] Integrated Genom, Chicago, IL 60612 USA

来源：

JOURNAL OF BACTERIOLOGY | 2002年 / 184卷 / 07期

关键词：

D O I：

10.1128/JB.184.7.2005-2018.2002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We present a complete DNA sequence and metabolic analysis of the dominant oral bacterium Fusobacterium nucleatum. Although not considered a major dental pathogen on its own, this anaerobe facilitates the aggregation and establishment of several other species including the dental pathogens Porphyromonas gingivalis and Bacteroides forsythus. The F. nucleatum strain ATCC 25586 genome was assembled from shotgun sequences and analyzed using the ERGO bioinformatics suite (http://Nvvviv.integratedgenomics.com). The genome contains 2.17 Mb encoding 2,067 open reading frames, organized on a single circular chromosome with 27% GC content. Despite its taxonomic position among the gram-negative bacteria, several features of its core metabolism are similar to that of grain-positive Clostridium spp., Enterococcus spp., and Lactococcus spp. The genome analysis has revealed several key aspects of (lie pathways of organic acid, amino acid, carbohydrate, and lipid metabolism. Nine very-high-molecular-weight outer membrane proteins are predicted from the sequence, none of which has been reported in the literature. More than 137 transporters for the uptake of a variety of substrates such as peptides, sugars, metal ions, and cofactors have been identified. Biosynthetic pathways exist for only three amino acids: glutamate, aspartate, and asparagine. The remaining amino acids are imported as such or as di- or oligopeptides that are subsequently degraded in the cytoplasm. A principal source of energy appears to be the fermentation of glutamate to butyrate. Additionally, desulfuration of cysteine and methionine yields ammonia, 11,S, methyl mereaptan, and butyrate, which are capable of arresting fibroblast growth, thus preventing wound healing and aiding penetration of the gingival epithelium. The metabolic capabilities of F. nucleatum, revealed by its genome are therefore consistent with its specialized niche in the mouth.

引用

页码：2005 / 2018

页数：14

共 46 条

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