Shear-flow-based chromatographic separations as an alternative to pressure-driven liquid chromatography

被引：25

作者：

Desmet, G ^{[1
]}

Vervoort, N ^{[1
]}

Clicq, D ^{[1
]}

Huau, A ^{[1
]}

Gzil, P ^{[1
]}

Baron, GV ^{[1
]}

机构：

[1] Free Univ Brussels, Dept Chem Engn, B-1050 Brussels, Belgium

来源：

JOURNAL OF CHROMATOGRAPHY A | 2002年 / 948卷 / 1-2期

关键词：

shear-driven chromatography; injection methods; detection; LC; computational fluid dynamics; instrumentation;

D O I：

10.1016/S0021-9673(01)01511-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

It is only by developing specially designed injection and detection systems that shear-driven chromatography can become a viable alternative to HPLC. In the present paper, a dedicated zero dead-volume injection procedure is presented with which sample volumes can be injected reproducibly in the required picoliter range. In addition, a transversal detection groove system is designed which should allow to perform on-line UV-VIS absorption measurements with path lengths in the millimeter range, with an acceptable theoretical plate loss (only 20% in a 5 cm long channel) and acting as a nearly perfect wave guide. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：19 / 34

页数：16

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