Sulfonamide resistance gene in a transferable R plasmid of Pasteurella piscicida

被引：13

作者：

Kim, EH ^{[1
]}

Aoki, T ^{[1
]}

机构：

[1] TOKYO UNIV FISHERIES,DEPT AQUAT BIOSCI,TOKYO 108,JAPAN

来源：

MICROBIOLOGY AND IMMUNOLOGY | 1996年 / 40卷 / 05期

关键词：

Pasteurella piscicida; sulfonamide resistance; pp-sul gene;

D O I：

10.1111/j.1348-0421.1996.tb01085.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The sulfonamide resistance (SA(r)) determinant was cloned from a transferable R plasmid of Pasteurella piscicida, pSP9351, and the sequence was determined. The resistance gene (pp-sul) was localized to an approximately 1-kb region that includes the PstI-EcoRI site in the restriction map. An open reading frame coding a sul II-type gene composed of 810 nucleotides was identified. A direct repeat sequence was shown in the 5' flanking region of pp-sul, and a plasmid recombinational event may have occurred during the construction of pSP9351. In the 3' flanking region of the gene, a sequence homologous to the 5' noncoding sequence of the trimethoprim resistance gene, dhfr IX was found.

引用

页码：397 / 399

页数：3

共 12 条

[1]

AOKI T, 1988, MICROBIOL SCI, V5, P219

[2]

Aoki T ., 1992, DIS ASIAN AQUA, P519

[3] SITE-SPECIFIC INSERTION OF GENE CASSETTES INTO INTEGRONS [J].