Molecular cloning and immunolocalization of a novel vertebrate trp homologue from Xenopus

被引：33

作者：

Bobanovic, LK

Laine, M

Petersen, CCH

Bennett, DL

Berridge, MJ

Lipp, P ^{[1
]}

Ripley, SJ

Bootman, MD

机构：

[1] Babraham Inst, Mol Signalling Lab, Cambridge CB2 3EJ, England

[2] Univ Cambridge, Dept Zool, Cambridge CB2 3EJ, England

来源：

BIOCHEMICAL JOURNAL | 1999年 / 340卷

基金：

英国惠康基金;

关键词：

D O I：

10.1042/0264-6021:3400593

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

We report the sequence, structure and distribution of a novel transient receptor potential (trp) homologue from Xenopus, Xtrp, determined by screening an oocyte cDNA library. On the basis of sequence similarity and predicted structure, Xtrp appears to be a homologue of mammalian trp1 proteins. Two polyclonal antibodies raised against distinct regions of the Xtrp sequence revealed Xtrp expression in various Xenopus tissues, and the localization of Xtrp at the plasma membrane of Xenopus oocytes and HeLa cells. Since capacitative calcium entry into Xenopus oocytes has been shown previously to be substantially inhibited by trp1 antisense oligonucleotides [Tomita, Kaneko, Funayama, Kondo, Satoh and Akaike (1998) Neurosci. Lett. 248, 195-198] we suggest that Xtrp may underlie capacitative calcium entry in Xenopus tissues.

引用

收藏

页码：593 / 599

页数：7

相关论文

共 41 条

[1]

INOSITOL TRISPHOSPHATE-INDUCED MEMBRANE-POTENTIAL OSCILLATIONS IN XENOPUS OOCYTES [J].

BERRIDGE, MJ .

JOURNAL OF PHYSIOLOGY-LONDON, 1988, 403 :589-599

[2]

INOSITOL TRISPHOSPHATE AND CALCIUM SIGNALING [J].

BERRIDGE, MJ .

NATURE, 1993, 361 (6410) :315-325

[3]

CAPACITATIVE CALCIUM-ENTRY [J].

BERRIDGE, MJ .

BIOCHEMICAL JOURNAL, 1995, 312 :1-11

[4]

On the molecular basis and regulation of cellular capacitative calcium entry: Roles for Trp proteins [J].

Birnbaumer, L ;

Zhu, X ;

Jiang, MS ;

Boulay, G ;

Peyton, M ;

Vannier, B ;

Brown, D ;

Platano, D ;

Sadeghi, H ;

Stefani, E ;

Birnbaumer, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (26) :15195-15202

[5]

Cloning and expression of a novel Mammalian homolog of Drosophila transient receptor potential (Trp) involved in calcium entry secondary to activation of receptors coupled by the G(q) class of G protein [J].

Boulay, G ;

Zhu, X ;

Peyton, M ;

Jiang, MS ;

Hurst, R ;

Stefani, E ;

Birnbaumer, L .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (47) :29672-29680

[6]

Hemispheric asymmetry of macroscopic and elementary calcium signals mediated by InsP3 in Xenopus oocytes [J].

Callamaras, N ;

Sun, XP ;

Ivorra, I ;

Parker, I .

JOURNAL OF PHYSIOLOGY-LONDON, 1998, 511 (02) :395-405

[7]

Concomitant and hormonally regulated expression of trp genes in bovine aortic endothelial cells [J].

Chang, AS ;

Chang, SM ;

Garcia, RL ;

Schilling, WP .

FEBS LETTERS, 1997, 415 (03) :335-340

[8]

MULTIPLE SEQUENCE ALIGNMENT WITH HIERARCHICAL-CLUSTERING [J].

CORPET, F .

NUCLEIC ACIDS RESEARCH, 1988, 16 (22) :10881-10890

[9]

Research report cloning and expression localization of cDNA for rat homolog of TRP protein, a possible store-operated calcium (Ca2+) channel [J].

Funayama, M ;

Goto, K ;

Kondo, H .

MOLECULAR BRAIN RESEARCH, 1996, 43 (1-2) :259-266

[10]

Differential expression of mammalian TRP homologues across tissues and cell lines [J].

Garcia, RL ;

Schilling, WP .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1997, 239 (01) :279-283

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