Relationship between CD107a expression and cytotoxic activity

被引：338

作者：

Aktas, Esin ^{[1
]}

Kucuksezer, Umut Can ^{[1
]}

Bilgic, Sema ^{[1
]}

Erten, Gaye ^{[1
]}

Deniz, Gunnur ^{[1
]}

机构：

[1] Istanbul Univ, Inst Expt Med DETAE, Dept Immunol, TR-34393 Istanbul, Turkey

来源：

CELLULAR IMMUNOLOGY | 2009年 / 254卷 / 02期

关键词：

NK cells; CD107a; Cylotoxicity; CFSE; IL-2; IFN-gamma; TNF-alpha; IL-10; NATURAL-KILLER-CELLS; HUMAN NK CELLS; SECRETORY LYSOSOMES; TARGET-CELLS; ASSAY; DIFFERENTIATION; IDENTIFICATION; GLYCOPROTEINS; ACTIVATION; CHEMOKINES;

D O I：

10.1016/j.cellimm.2008.08.007

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

NK cells play important roles in innate immunity against tumors and infections of the host. Studies show that CD107a (LAMP-1) may be a marker for degranulation of NK and activated CD8(+) T cells. In our study, the relationship between the expression of CD107a, cytokine secretion and cytotoxic activity in CD56(+) NK, CD8(+) T cells and lymphocytes has been determined after various stimuli. Effector cells from PBMCs of healthy subjects were isolated and K562 cell line was used as target of cytotoxicity. IL-2 stimulation resulted in a significant increase of CD107a expression in CID56(+) NK, CD8(+) T cells and lymphocytes. Increased expression of CD107a after IL-2 stimulation of NK cells was parallel to the increase of cytotoxicity. Our results suggest that CD107a expression may be a sensitive marker for the cytotoxic activity determination. (c) 2008 Elsevier Inc. All rights reserved.

引用

页码：149 / 154

页数：6

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