Cell-associated collagenolytic activity by Candida albicans

被引：15

作者：

Nishimura, M

Nikawa, H

Yamashiro, H

Nishimura, H

Hamada, T

Embery, G

机构：

[1] Hiroshima Univ, Fac Dent, Dept Prosthet Dent, Minami Ku, Hiroshima 7348553, Japan

[2] Univ Wales Coll Cardiff, Coll Med, Sch Dent, Dept Basic Dent Sci, Cardiff CF14 4XY, S Glam, Wales

来源：

MYCOPATHOLOGIA | 2002年 / 153卷 / 03期

关键词：

Candida albicans; collagenolytic activity; root caries;

D O I：

10.1023/A:1014506115757

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Cell associated collagenolytic activity of Candida albicans was quantified by measuring the degradation of synthetic peptide 2-furanacryloyl-Leu-Gly-Pro-Ala (FALGPA), which is a specific substrate for collagenase, by the freeze-thaw procedure method. This collagenolytic activity was enhanced by cells cultured in the presence of bovine serum albumin (BSA) in culture medium. However, this activity was inhibited in the presence of ethylenediaminetetraacetic acid disodium salt (EDTA-2Na), but not by the serine proteinase inhibitor p-amidinophenyl methanesulfonyl fluoride (APMSF), nor the aspartyl proteinase inhibitor pepstatin A. These results suggested the presence of a metalloenzyme on pericellular C. albicans.

引用

页码：125 / 128

页数：4

共 22 条

[1] USE OF CHROMAGAR CANDIDA MEDIUM FOR ISOLATION OF YEASTS FROM DENTAL SAMPLES [J].