Combinatorial effects of conception and governor vessel electroacupuncture and human umbilical cord blood-derived mesenchymal stem cells on pathomorphologic lesion and cellular apoptosis in rats with cerebral ischemia/reperfusion

被引：20

作者：

Yang, Zhuoxin ^{[1
]}

Chen, Pengdian ^{[2
]}

Yu, Haibo ^{[2
]}

Luo, Wenshu ^{[2
]}

Pi, Min ^{[2
]}

Wu, Yonggang ^{[2
]}

Wang, Ling ^{[2
]}

Yang, Fuxia ^{[2
]}

Gou, Yanhua ^{[2
]}

机构：

[1] Southern Med Univ, Affiliated Shenzhen Matern & Child Healthcare Hos, Dept Tradit Chinese Med, Shenzhen 518028, Peoples R China

[2] Guangzhou Univ Chinese Med, Affiliated Shenzhen Tradit Chinese Med Hosp, Dept Acupuncture & Moxibust, Shenzhen 518033, Peoples R China

来源：

JOURNAL OF TRADITIONAL CHINESE MEDICINE | 2013年 / 33卷 / 06期

基金：

中国国家自然科学基金;

关键词：

Electroacupuncture; Conception vessel; Governor vessel; Fetal blood; Mesenchymal stem cells; Brain ischemia; Apoptosis; DNA FRAGMENTATION; ISCHEMIA; TRANSPLANTATION; RECOVERY; MODEL; PROLIFERATION; ACUPUNCTURE; STROKE; INJURY;

D O I：

10.1016/S0254-6272(14)60012-1

中图分类号：

R [医药、卫生];

学科分类号：

100218 [急诊医学];

摘要：

OBJECTIVE: To investigate the combinatorial effects of conception and governor vessel electroacupuncture (EA) and human umbilical cord blood-derived mesenchymal stem cells (HUCB-MSCs) on pathomorphologic lesion and cellular apoptosis in rats with cerebral ischemia/reperfusion. METHODS: With the HUCB-MSCs isolated, cultured and identified and the models of cerebral ischemia-reperfusion established, the HUCB-MSCs of passage three were intracranially transplanted and the EA at conception and governor vessels was applied. The pathomorphologic lesion by hematoxylin-eosin staining and the cellular apoptosis by terminal deoxynucleotidyl transferase-mediated nick-end labeling method around the ischemic focus were observed. RESULTS: The cultured adherent HUCB-MSCs exhibited a spindle shape and expressed MSC-specific markers, with the cell purity and proliferation rate significantly increasing after the primary passage. HE staining showed that there were no pathological changes observed in the sham surgery group. However, in the PBS transplantation group, degeneration and necrosis of a great number of nerve cells were seen. In both the HUCB-MSCs transplantation group and the HUCB-MSCs transplantation + EA group, reparative changes of the pathomorphism of the tissue were found. Both combination treatment and simple MSCs treatment were able to improve the pathomorphorlogic lesion following cerebral ischemia and reduce the abnormal TUNEL-positive numbers, with former better than latter. CONCLUSION: HUCB-MSCs improve pathological lesions and inhibit the cellular apoptosis around the cerebral ischemic area. EA at conception and governor vessels also improve pathological lesion and inhibit the cellular apoptosis in rats treated with HUCB-MSCs transplantation, which effects were superior to that of simple HUCB-MSCs transplantation. (C) 2013 JTCM. All rights reserved.

引用

页码：779 / 786

页数：8

共 29 条

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