Piggy-BACing the human genome - II. A high-resolution, physically anchored, comparative map of the porcine autosomes

被引：93

作者：

Meyers, SN

Rogatcheva, MB

Larkin, DM

Yerle, M

Milan, D

Hawken, RJ

Schook, LB

Beever, JE

机构：

[1] Univ Illinois, Urbana, IL 61801 USA

[2] INRA, Lab Genet Cellulaire, F-31931 Toulouse, France

来源：

GENOMICS | 2005年 / 86卷 / 06期

关键词：

comparative mapping; radiation hybrid; BAC; porcine;

D O I：

10.1016/j.ygeno.2005.04.010

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Using the INRA-Minnesota porcine radiation hybrid panel, we have constructed a human-pig comparative map composed of 2274 loci, including 206 ESTs and 2068 BAC-end sequences, assigned to 34 linkage groups. The average spacing between comparative anchor loci is 1.15 Mb based on human genome sequence coordinates. A total of 51 conserved synteny groups that include 173 conserved segments were identified. This radiation hybrid map has the highest resolution of any porcine map to date and its integration with the porcine linkage map (reported here) will greatly facilitate the positional cloning of genes influencing complex traits of both agricultural and biomedical interest. Additionally, this map will provide a framework for anchoring contigs generated through BAC fingerprinting efforts and assist in the selection of a BAC minimal tiling path and assembly of the first sequence-ready map of the porcine genome. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：739 / +

页数：12

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