Brassinosteroids regulate grain filling in rice

被引：307

作者：

Wu, Chuan-yin ^{[1
]}

Trieu, Anthony ^{[1
]}

Radhakrishnan, Parthiban ^{[1
]}

Kwok, Shing F. ^{[1
]}

Harris, Sam ^{[1
]}

Zhang, Ke ^{[1
]}

Wang, Jiulin ^{[2
]}

Wan, Jianmin ^{[2
]}

Zhai, Huqu ^{[2
]}

Takatsuto, Suguru ^{[3
]}

Matsumoto, Shogo ^{[4
]}

Fujioka, Shozo ^{[4
]}

Feldmann, Kenneth A. ^{[1
]}

Pennell, Roger I. ^{[1
]}

机构：

[1] Ceres Inc, Thousand Oaks, CA 91320 USA

[2] Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China

[3] Joetsu Univ Educ, Dept Chem, Joetsu, Niigata 9438512, Japan

[4] RIKEN, Inst Phys & Chem Res, Adv Sci Inst, Wako, Saitama 3510198, Japan

来源：

PLANT CELL | 2008年 / 20卷 / 08期

关键词：

D O I：

10.1105/tpc.107.055087

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Genes controlling hormone levels have been used to increase grain yields in wheat (Triticum aestivum) and rice (Oryza sativa). We created transgenic rice plants expressing maize (Zea mays), rice, or Arabidopsis thaliana genes encoding sterol C-22 hydroxylases that control brassinosteroid (BR) hormone levels using a promoter that is active in only the stems, leaves, and roots. The transgenic plants produced more tillers and more seed than wild-type plants. The seed were heavier as well, especially the seed at the bases of the spikes that fill the least. These phenotypic changes brought about 15 to 44% increases in grain yield per plant relative to wild-type plants in greenhouse and field trials. Expression of the Arabidopsis C-22 hydroxylase in the embryos or endosperms themselves had no apparent effect on seed weight. These results suggested that BRs stimulate the flow of assimilate from the source to the sink. Microarray and photosynthesis analysis of transgenic plants revealed evidence of enhanced CO2 assimilation, enlarged glucose pools in the flag leaves, and increased assimilation of glucose to starch in the seed. These results further suggested that BRs stimulate the flow of assimilate. Plants have not been bred directly for seed filling traits, suggesting that genes that control seed filling could be used to further increase grain yield in crop plants.

引用

页码：2130 / 2145

页数：16

共 69 条

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