Pyrolysis Mass Spectrometry (PyMS) and 16S-23S rDNA spacer region fingerprinting suggests the presence of novel acinetobacters in activated sludge

被引:5
作者
Carr, E
Ward, A
Gurtler, V
Seviour, RJ
机构
[1] La Trobe Univ, Biotechnol Res Ctr, Bendigo, Vic 3550, Australia
[2] Univ Newcastle Upon Tyne, Dept Agr Sci, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England
[3] Austin & Repatriat Hosp, Dept Microbiol, Melbourne, Vic, Australia
关键词
activated sludge; Acinetobacter; 16S-23S spacer region; pyrolysis mass spectrometry; biological phosphorus removal;
D O I
10.1078/0723-2020-00039
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Screening of large numbers of Acinetobacter spp. from activated sludge systems with Pyrolysis Mass Spectrometry (PyMS) showed that many did not cluster tightly with the currently described genomic species which have been obtained mainly from clinical sources. Selected isolates were then genotypically fingerprinted using their 16S-23S rDNA spacer region, and again the data revealed considerable differences in the genomic fingerprints of many of these activated sludge isolates to the predominantly clinical genomic species. In fact, few could be identified from them. The possibility that the current speciation within this genus is not adequate to encompass all these environmental isolates is addressed in relation to the methods used to study the population dynamics of Acinetobacter in activated sludge.
引用
收藏
页码:430 / 442
页数:13
相关论文
共 56 条