Bone marrow-derived mononuclear cells vs. mesenchymal stromal cells in experimental allergic asthma

被引：56

作者：

Abreu, Soraia C. ^{[1
]}

Antunes, Mariana A. ^{[1
]}

de Castro, Julia C. ^{[1
]}

de Oliveira, Milena V. ^{[1
]}

Bandeira, Elga ^{[1
]}

Ornellas, Debora S. ^{[1
]}

Diaz, Bruno L. ^{[2
]}

Morales, Marcelo M. ^{[3
]}

Xisto, Debora G. ^{[1
,3
]}

Rocco, Patricia R. M. ^{[1
]}

机构：

[1] Univ Fed Rio de Janeiro, Carlos Chagas Filho Inst Biophys, Lab Pulm Invest, BR-21941902 Rio De Janeiro, RJ, Brazil

[2] Univ Fed Rio de Janeiro, Carlos Chagas Filho Inst Biophys, Intermediate Lab Inflammat, BR-21941902 Rio De Janeiro, RJ, Brazil

[3] Univ Fed Rio de Janeiro, Carlos Chagas Filho Inst Biophys, Lab Cellular & Mol Physiol, BR-21941902 Rio De Janeiro, RJ, Brazil

来源：

RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY | 2013年 / 187卷 / 02期

关键词：

Asthma; Allergic lung disease; Airway epithelium; Pulmonary eosinophilia; STEM-CELLS; MURINE MODEL; MOUSE MODEL; LUNG; AIRWAY; INFLAMMATION; EXPRESSION; SUPPRESSION; FIBROSIS; THERAPY;

D O I：

10.1016/j.resp.2013.03.014

中图分类号：

Q4 [生理学];

学科分类号：

071003 [生理学];

摘要：

We compared the effects of bone marrow-derived mononuclear cells (BMMCs) and mesenchymal stromal cells (MSCs) on airway inflammation and remodeling and lung mechanics in experimental allergic asthma. C57BL/6 mice were sensitized and challenged with ovalbumin (OVA group). A control group received saline using the same protocol. Twenty-four hours after the last challenge, groups were further randomized into subgroups to receive saline, BMMCs (2 x 10(6)) or MSCs (1 x 10(5)) intratracheally. BMMC and MSC administration decreased cell infiltration, bronchoconstriction index, alveolar collapse, collagen fiber content in the alveolar septa, and interleukin (IL)-4, IL-13, transforming growth factor (TGF)-beta and vascular endothelial growth factor (VEGF) levels compared to OVA-SAL Lung function, alveolar collapse, collagen fiber deposition in alveolar septa, and levels of TGF-beta and VEGF improved more after BMMC than MSC therapy. In conclusion, intratracheal BMMC and MSC administration effectively modulated inflammation and fibrogenesis in an experimental model of asthma, but BMMCs was associated with greater benefit in terms of reducing levels of fibrogenesis-related growth factors. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：190 / 198

页数：9

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