Temporal profile of CREB phosphorylation after focal ischemia in rat brain

被引：41

作者：

Tanaka, K ^{[1
]}

Nogawa, S ^{[1
]}

Nagata, E ^{[1
]}

Suzuki, S ^{[1
]}

Dembo, T ^{[1
]}

Kosakai, A ^{[1
]}

Fukuuchi, Y ^{[1
]}

机构：

[1] Keio Univ, Sch Med, Dept Neurol, Shinjuku Ku, Tokyo 1608582, Japan

来源：

NEUROREPORT | 1999年 / 10卷 / 11期

关键词：

cAMP response element binding protein; cerebral ischemia; CREB; middle cerebral artery; phosphorylation; signal transduction;

D O I：

10.1097/00001756-199908020-00004

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

THE phosphorylation of cAMP response element binding protein (CREB) in the rat brain was examined immunohistochemically at 3.5 h, 12 h, 24 h and 48 h of recirculation after focal ischemia induced by occlusion of the middle cerebral artery for 1.5 h. Brain sections were stained with affinity purified anti-phosphorylated CREB antibody. The ischemic core revealed a significant, but transient increase in number of phosphorylated CREB-positive cells at 3.5 h of recirculation, followed by a rapid decrease during the subsequent period. In the peri-ischemia area, the number of phosphorylated CREB-positive cells showed a more marked increase as compared to that in the ischemic core at 3.5 h of recirculation, and the increase continued until 48 h of recirculation with a tendency for gradual decline. Persistent enhancement of CREB phosphorylation may thus be closely related to the neuronal viability and neuroprotective mechanisms, whereas rapid disappearance of CREB phosphorylation may clearly precede neuronal death. NeuroReport 10:2245-2250 (C) 1999 Lippincott Williams & Wilkins.

引用

页码：2245 / 2250

页数：6

共 25 条

[1] EXPRESSION OF C-FOS AND C-JUN FAMILY GENES AFTER FOCAL CEREBRAL-ISCHEMIA [J].

AN, G ;

LIN, TN ;

LIU, JS ;

XUE, JJ ;

HE, YY ;

HSU, CY .

ANNALS OF NEUROLOGY, 1993, 33 (05) :457-464

[2] RAT MIDDLE CEREBRAL-ARTERY OCCLUSION - EVALUATION OF THE MODEL AND DEVELOPMENT OF A NEUROLOGIC EXAMINATION [J].

BEDERSON, JB ;

PITTS, LH ;

TSUJI, M ;

NISHIMURA, MC ;

DAVIS, RL ;

BARTKOWSKI, H .

STROKE, 1986, 17 (03) :472-476

[3] Hypoxia induces phosphorylation of the cyclic AMP response element-binding protein by a novel signaling mechanism [J].

Beitner-Johnson, D ;

Millhorn, DE .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (31) :19834-19839

[4] Middle cerebral artery occlusion in the rat by intraluminal suture - Neurological and pathological evaluation of an improved model [J].

Belayev, L ;

Alonso, OF ;

Busto, R ;

Zhao, WZ ;

Ginsberg, MD .

STROKE, 1996, 27 (09) :1616-1622

[5] FOCAL ISCHEMIA CAUSES AN EXTENSIVE INDUCTION OF IMMEDIATE-EARLY GENES THAT ARE SENSITIVE TO MK-801 [J].

COLLACOMORAES, Y ;

ASPEY, BS ;

DEBELLEROCHE, JS ;

HARRISON, MJG .

STROKE, 1994, 25 (09) :1855-1860

[6] Rsk-2 activity is necessary for epidermal growth factor-induced phosphorylation of CREB protein and transcription of c-fos gene [J].

De Cesare, D ;

Jacquot, S ;

Hanauer, A ;

Sassone-Corsi, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (21) :12202-12207

[7] CREB: A major mediator of neuronal neurotrophin responses [J].

Finkbeiner, S ;

Tavazoie, SF ;

Maloratsky, A ;

Jacobs, KM ;

Harris, KM ;

Greenberg, ME .

NEURON, 1997, 19 (05) :1031-1047

[8] REGULATION OF CREB PHOSPHORYLATION IN THE SUPRACHIASMATIC NUCLEUS BY LIGHT AND A CIRCADIAN CLOCK [J].

GINTY, DD ;

KORNHAUSER, JM ;

THOMPSON, MA ;

BADING, H ;

MAYO, KE ;

TAKAHASHI, JS ;

GREENBERG, ME .

SCIENCE, 1993, 260 (5105) :238-241

[9] NERVE GROWTH-FACTOR ACTIVATES A RAS-DEPENDENT PROTEIN-KINASE THAT STIMULATES C-FOS TRANSCRIPTION PHOSPHORYLATION OF CREB [J].

GINTY, DD ;

BONNI, A ;

GREENBERG, ME .

CELL, 1994, 77 (05) :713-725

[10] Attenuated c-fos mRNA induction after middle cerebral artery occlusion in CREB knockout mice does not modulate focal ischemic injury [J].

Hata, R ;

Gass, P ;

Mies, G ;

Wiessner, C ;

Hossmann, KA .

JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM, 1998, 18 (12) :1325-1335

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