Group II introns as controllable gene targeting vectors for genetic manipulation of bacteria

被引：153

作者：

Karberg, M

Guo, HT

Zhong, J

Coon, R

Perutka, J

Lambowitz, AM ^{[1
]}

机构：

[1] Univ Texas, Dept Chem & Biochem, Inst Mol & Cellular Biol, Austin, TX 78712 USA

[2] Univ Texas, Sch Biol Sci, Sect Mol Genet & Microbiol, Austin, TX 78712 USA

来源：

NATURE BIOTECHNOLOGY | 2001年 / 19卷 / 12期

关键词：

D O I：

10.1038/nbt1201-1162

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Mobile group II introns can be retargeted to insert into virtually any desired DNA target. Here we show that retargeted group II introns can be used for highly specific chromosomal gene disruption in Escherichia coli and other bacteria at frequencies of 0.1-22%. Furthermore, the introns can be used to introduce targeted chromosomal breaks, which can be repaired by transformation with a homologous DNA fragment, enabling the introduction of point mutations. Because of their wide host range, mobile group II introns should be useful for genetic engineering and functional genomics in a wide variety of bacteria.

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页码：1162 / 1167

页数：6

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