Encapsulation of Organic Molecules in Calcium Phosphate Nanocomposite Particles for Intracellular Imaging and Drug Delivery

被引：239

作者：

Morgan, Thomas T. ^{[1
]}

Muddana, Hari S. ^{[2
]}

Altinoglu, Erhan I. ^{[1
]}

Rouse, Sarah M. ^{[1
]}

Tabakovic, Amra ^{[1
]}

Tabouillot, Tristan ^{[2
]}

Russin, Timothy J. ^{[1
,3
]}

Shanmugavelandy, Sriram S. ^{[4
]}

Butler, Peter J. ^{[2
]}

Eklund, Peter C. ^{[1
,3
]}

Yun, Jong K. ^{[4
]}

Kester, Mark ^{[4
]}

Adair, James H. ^{[1
]}

机构：

[1] Penn State Univ, Dept Mat Sci & Engn, University Pk, PA 16802 USA

[2] Penn State Univ, Dept Bioengn, University Pk, PA 16802 USA

[3] Penn State Univ, Dept Engn, University Pk, PA 16802 USA

[4] Milton S Hershey Med Ctr, Dept Pharmacol, Hershey, PA 17033 USA

来源：

NANO LETTERS | 2008年 / 8卷 / 12期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1021/nl8019888

中图分类号：

O6 [化学];

学科分类号：

0703 [化学];

摘要：

Encapsulation of imaging agents and drugs in calcium phosphate nanoparticles (CPNPs) has potential as a nontoxic, bioresorbable vehicle for drug delivery to cells and tumors. The objectives of this study were to develop a calcium phosphate nanoparticle encapsulation system for organic dyes and therapeutic drugs so that advanced fluoresence methods could be used to assess the efficiency of drug delivery and possible mechanisms of nanoparticle bioabsorption. Highly concentrated CPNPs encapsulating a variety of organic fluorophores were successfully synthesized. Well-dispersed CPNPs encapsulating Cy3 amidite exhibited nearly a 5-fold increase in fluorescence quantum yield when compared to the free dye in PBS. FCS diffusion data and cell staining were used to show pH-dependent dissolution of the particles and cellular uptake, respectively. Furthermore, an experimental hydrophobic cell growth inhibitor, ceramide, was successfully delivered in vitro to human vascular smooth muscle cells via encapsulation in CPNPs. These studies demonstrate that CPNPs are effective carriers of dyes and drugs for bioimaging and, potentially, for therapeutic intervention.

引用

页码：4108 / 4115

页数：8

共 42 条

[1]

ADAIR JH, 2004, LESSONS NANOTECHNOLO, P93

[2]

ADAIR JH, 1995, UROLITHIASIS, V2, P181

[3]

Alberts B., 2002, Molecular Biology of The Cell, V4th

[4]

ALTMOGLU EI, 2008, NEAR INFRARED EMITTI

[5]

THE NATURE OF THE SILICA CAGE AS REFLECTED BY SPECTRAL CHANGES AND ENHANCED PHOTOSTABILITY OF TRAPPED RHODAMINE-6G [J].

AVNIR, D ;

LEVY, D ;

REISFELD, R .

JOURNAL OF PHYSICAL CHEMISTRY, 1984, 88 (24) :5956-5959

[6]

Optimization of dye-doped silica nanoparticles prepared using a reverse microemulsion method [J].

Bagwe, RP ;

Yang, CY ;

Hilliard, LR ;

Tan, WH .

LANGMUIR, 2004, 20 (19) :8336-8342

[7]

Preparation and flow cytometry of uniform silica-fluorescent dye microspheres [J].

Bele, M ;

Siiman, O ;

Matijevic, E .

JOURNAL OF COLLOID AND INTERFACE SCIENCE, 2002, 254 (02) :274-282

[8]

pDNA loaded calcium phosphate nanoparticles: highly efficient non-viral vector for gene delivery [J].

Bisht, S ;

Bhakta, G ;

Mitra, S ;

Maitra, A .

INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2005, 288 (01) :157-168

[9]

Ceramide-induced inhibition of Akt is mediated through protein kinase Cζ -: Implications for growth arrest [J].

Bourbon, NA ;

Sandirasegarane, L ;

Kester, M .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (05) :3286-3292

[10]

Ceramide-coated balloon catheters limit neointimal hyperplasia after stretch injury in carotid arteries [J].

Charles, R ;

Sandirasegarane, L ;

Yun, J ;

Bourbon, N ;

Wilson, R ;

Rothstein, RP ;

Levison, SW ;

Kester, M .

CIRCULATION RESEARCH, 2000, 87 (04) :282-288

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