Association of a BMP9 Haplotype with Ossification of the Posterior Longitudinal Ligament (OPLL) in a Chinese Population

被引:36
作者
Ren, Yuan [1 ]
Liu, Zhi-zhong [2 ]
Feng, Jie [3 ]
Wan, Hong [3 ]
Li, Jun-hua [3 ]
Wang, Hao [1 ]
Lin, Xin [1 ]
机构
[1] Capital Med Univ, Dept Orthopaed, Beijing Tiantan Hosp, Beijing, Peoples R China
[2] Capital Med Univ, Dept Clin Lab, Beijing Tiantan Hosp, Beijing, Peoples R China
[3] Capital Med Univ, Dept Inst Neurosurg, Beijing Tiantan Hosp, Beijing, Peoples R China
基金
中国国家自然科学基金; 北京市自然科学基金;
关键词
BONE MORPHOGENETIC PROTEIN-2; MESENCHYMAL STEM-CELLS; BODY-MASS INDEX; CERVICAL-SPINE; GENE-THERAPY; POLYMORPHISMS; RISK; SUSCEPTIBILITY; JAPAN;
D O I
10.1371/journal.pone.0040587
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];
摘要
Direct or ex vivo BMP9 adenoviral gene therapy can induce massive bone formation at the injection sites and clearly promote spinal fusion. A comprehensive analysis of the osteogenic activity indicated that BMP9 was one of the most potent inducers of osteogenic differentiation both in vitro and in vivo among 14 types of human BMPs. However, genetic variations and whether they correlated with OPLL were not considered. We have sequenced the complete BMP9 gene in 450 patients with OPLL and in 550 matched controls. Analyses were performed on single markers and haplotypes. Single marker tests identified 6 SNPs, among which the minor alleles of rs7923671 (T > C; P = 0.0026; OR: 1.33, CI: 1.10-1.60), rs75024165 (C > T, Thr304Met; P < 0.001; OR: 1.76, CI: 1.47-2.12) and rs34379100 (A > C; P < 0.001; OR: 1.52, CI: 1.27-1.82) were associated with OPLL. Logistic regression analysis showed that the additive model of rs75024165 (TT vs. CT vs. CC; P < 0.001; OR: 1.74) and rs34379100 (CC vs. AC vs. AA; P = 0.003; OR: 1.95) retained statistical significance when adjusted for clinical and demographic characteristics. Linkage disequilibrium (LD) analysis identified one 3 kb block of intense LD in BMP9 and one specific haplotype, CTCA (P < 0.001; OR: 2.37), that contained the OPLL-associated risk alleles and was a risk factor for OPLL. This haplotype is associated with increased severity of OPLL, as shown by the distribution of ossified vertebrae in patients with OPLL (P = 0.001). In summary, in the Chinese population studied, SNPs in the BMP9 gene appear to contribute to the risk of OPLL in association with certain clinical and demographic characteristics. The severity of OPLL seems to be mediated predominantly by genetic variations in a 3kb BMP9 locus with the specific haplotype CTCA.
引用
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页数:7
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