Matrilysin stimulates DNA synthesis of cultured vascular endothelial cells and induces angiogenesis in vivo

被引：55

作者：

Nishizuka, I

Ichikawa, Y

Ishikawa, T

Kamiyama, M

Hasegawa, S

Momiyama, N

Miyazaki, K

Shimada, H

机构：

[1] Yokohama City Univ, Sch Med, Dept Surg 2, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan

[2] Yokohama City Univ, Div Cell Biol, Kihara Inst Biol Res, Yokohama, Kanagawa 232, Japan

来源：

CANCER LETTERS | 2001年 / 173卷 / 02期

关键词：

matrilysin; vascular endothelial cells; angiogenesis; in vivo;

D O I：

10.1016/S0304-3835(01)00634-6

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Matrilysin produced by human colon cancer cells may be involved in the progression and metastasis of cancer. In the present study, we investigated the association of matrilysin with angiogenesis. One microgram of recombinant matrilysin is confirmed to have increased [H-3]-thymidine uptake in human umbilical vein endothelial cells. Then we used micro encapsulation and a mouse hemoglobin enzyme-linked immunosorbent assay system for in vivo quantitation of angiogenesis with BALB/c nu/nu athymic mice. Hundred micrograms of recombinant matrilysin induced angiogenesis to the same degree as 10 mug of basic fibroblast growth factor (bFGF). Angiogenesis was observed at the site implanted with human colon cancer WiDr cells in agarose micro beads. This was inhibited by subcutaneous injection of matrilysin-specific antisense oligonucleotide significantly by 53%. In conclusion, matrilysin may be associated with angiogenesis of human colon cancer through the direct proliferative action on endothelial cells. (C) 2001 Published by Elsevier Science Ireland Ltd.

引用

页码：175 / 182

页数：8

共 28 条

[1] Diverse cell surface protein ectodomains are shed by a system sensitive to metalloprotease inhibitors [J].