Fluorescent monitoring of kinase activity in real time: Development of a robust fluorescence-based assay for abi tyrosine kinase activity

被引：22

作者：

Hofmann, RM

Cotton, GJ

Chang, EJ

Vidal, E

Veach, D

Bornmann, W

Muir, TW

机构：

[1] Rockefeller Univ, Lab Synthet Prot Chem, New York, NY 10021 USA

[2] Mem Sloan Kettering Canc Ctr, Organ Synth Core, New York, NY 10021 USA

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2001年 / 11卷 / 24期

关键词：

D O I：

10.1016/S0960-894X(01)00650-3

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Fluorescent biosensors hold great promise for drug discovery. Using a solid-phase version of protein semi-synthesis. we incorporated two fluorophores at specific sites within a truncated version of the c-Crk-II protein. The resulting fluorescent protein biosensor permits the real-time monitoring of Abl kinase activity and provides a robust and rapid method for assaying Abl kinase inhibitors. (C) 2001 Elsevier Science Ltd. All rights reserved.

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收藏

页码：3091 / 3094

页数：4

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