Studying Protein Structure and Function Using Semisynthesis

被引：15

作者：

Muir, Tom W. ^{[1
]}

机构：

[1] Rockefeller Univ, Lab Synthet Prot Chem, New York, NY 10021 USA

来源：

BIOPOLYMERS | 2008年 / 90卷 / 06期

基金：

美国国家卫生研究院;

关键词：

du Vigneaud; protein chemistry; award;

D O I：

10.1002/bip.21102

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this review, which is more or less a transcript of my du Vigneaud Award Lecture, I cover the development and application of the protein semisynthesis technique, Expressed Protein Ligation (EPL). EPL allows the assembly of modified proteins from recombinant and synthetic peptide building blocks. The approach has been widely used since its introduction in 1998 and has allowed a number of biochemical problems to be solved through the use of OEdesigner(1) proteins. In this article, the utility of the approach is illustrated through work in my own lab and with an emphasis on the use of EPL to study the role of protein post-translational modifications (C) 2008 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 90: 743-750, 2008.

引用

页码：743 / 750

页数：8

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