Development of microsatellite markers in the Japanese pear (Pyrus pyrifolia Nakai)

被引：166

作者：

Yamamoto, T ^{[1
]}

Kimura, T

Shoda, M

Ban, Y

Hayashi, T

Matsuta, N

机构：

[1] Natl Inst Fruit Tree Sci, Dept Breeding, Tsukuba, Ibaraki 3058605, Japan

[2] Natl Ctr Seeds & Seedlings, Tsukuba, Ibaraki 3050852, Japan

来源：

MOLECULAR ECOLOGY NOTES | 2002年 / 2卷 / 01期

关键词：

cross-species amplification; enriched library; microsatellite; pear;

D O I：

10.1046/j.1471-8286.2002.00128.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Thirteen polymorphic microsatellite loci were developed in the Japanese pear (Pyrus pyrifolia Nakai) by using an enriched genomic library. The obtained microsatellite loci showed a high degree of polymorphism in the Japanese pear with 3-6 alleles per locus. The average values of observed and expected heterozygosities among these 13 loci were 0.69 and 0.71, respectively. Ten microsatellites could successfully amplify loci in the European pear (Pyrus communis L.), which were highly polymorphic as well.

引用

页码：14 / 16

页数：3