The priming action of tumour necrosis factor-alpha (TNF-alpha) and granulocyte-macrophage colony-stimulating factor (GM-CSF) on neutrophils activated by inflammatory microcrystals

被引：17

作者：

Burt, HM

Jackson, JK

机构：

[1] Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, BC

[2] Faculty of Pharmaceutical Sciences, 2146 East Mall, University of British Columbia, Vancouver

来源：

CLINICAL AND EXPERIMENTAL IMMUNOLOGY | 1997年 / 108卷 / 03期

关键词：

monosodium urate monohydrate; calcium pyrophosphate dihydrate; tumour necrosis; factor-alpha; granulocyte-macrophage colony-stimulating factor; neutrophils;

D O I：

10.1046/j.1365-2249.1997.4011298.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We studied the effects of TNF-alpha or GM-CSF on the production of reactive oxygen species (as measured by chemiluminescence) and degranulation responses of neutrophils to opsonized inflammatory microcrystals. TNF-alpha in the 10-2000 pM or GM-CSF in the 2-200 pM concentration range caused the concentration-dependent amplification of neutrophil chemiluminescence responses to both calcium pyrophosphate dihydrate (CPPD) and monosodium mate monohydrate (MSUM) crystals. Degranulation responses, as measured by the extracellular release of the granule enzymes myeloperoxidase or lysozyme, were amplified by approximate to 50-100% for both MSUM or CPPD crystal-induced neutrophil activation when cells were pretreated with TNF-alpha at 2000 pM or GM-CSF at 75 pM.

引用

页码：432 / 437

页数：6

共 53 条

[1] ROLE OF TYROSYL PHOSPHORYLATION IN NEUTROPHIL PRIMING BY TUMOR-NECROSIS-FACTOR-ALPHA AND GRANULOCYTE COLONY STIMULATING FACTOR [J].