Anti-apoptotic Function and Mechanism of Ginseng Saponins in Rattus Pancreatic β-Cells

被引：37

作者：

Chen, Fang ^{[1
,2
]}

Chen, Yuewen ^{[1
,2
]}

Kang, Xincong ^{[2
]}

Zhou, Zhiguang ^{[4
]}

Zhang, Zhixu ^{[2
,3
]}

Liu, Dongbo ^{[1
,2
,3
]}

机构：

[1] Hunan Agr Univ, Hort & Landscape Coll, Changsha 410128, Hunan, Peoples R China

[2] State Adm Tradit Chinese Med, State Key Lab Sub Hlth Intervent Technol, Changsha 410128, Hunan, Peoples R China

[3] Key Lab Crop Germplasm Innovat & Utilizat Hunan P, Changsha 410128, Hunan, Peoples R China

[4] Cent S Univ, Ctr Diabet, Inst Metab & Endocrinol, Changsha 410011, Hunan, Peoples R China

来源：

BIOLOGICAL & PHARMACEUTICAL BULLETIN | 2012年 / 35卷 / 09期

关键词：

apoptosis; ginsenoside; high glucose; cytokine; diabetes; DEPENDENT DIABETES-MELLITUS; CHOP GADD153; NITRIC-OXIDE; HUMAN ISLETS; IN-VITRO; DEATH; INSULIN; EXPRESSION; TYPE-1; RADIX;

D O I：

10.1248/bpb.b12-00461

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Apoptosis is the main form of beta-cell death in diabetes. Ginseng has been used as an anti-diabetic herb for several thousand years in Asia with ginsenoside Rg1 and ginsenoside Rb1 as important active ingredients. In this study, we demonstrated ginsenoside Rg1 and Rb1 protect beta-cells from high glucose/cytokine-induced pancreatic beta-cell apoptosis via inhibiting nitric oxide (NO) production and regulating apoptosis-related genes. Among these genes, Bax, Fas and Caspase-3 gene expressions were up-regulated by high glucose, whereas only Bax and Caspase-3 gene expression were elevated by cytokines. In these two stimuli-induced apoptotic cells, Rg1 down-regulated Fas gene expression, while Rb1 decreased Caspase-3 gene expression. As a conclusion, Fas signal pathway and mitochondria stress is mostly related to anti-diabetes function of ginsenoside Rg1, while Caspase-3 pathway is essential when Rb1 is present.

引用

页码：1568 / 1573

页数：6

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