RETRACTED: Generation of pluripotent stem cells from adult human testis (Retracted article. See vol. 512, pg. 338, 2014)

被引：347

作者：

Conrad, Sabine ^{[1
]}

Renninger, Markus ^{[3
]}

Hennenlotter, Joerg ^{[3
]}

Wiesner, Tina ^{[1
]}

Just, Lothar ^{[1
]}

Bonin, Michael ^{[4
]}

Aicher, Wilhelm ^{[5
,6
]}

Buehring, Hans-Joerg ^{[7
]}

Mattheus, Ulrich ^{[2
]}

Mack, Andreas ^{[2
]}

Wagner, Hans-Joachim ^{[2
]}

Minger, Stephen ^{[8
]}

Matzkies, Matthias ^{[9
]}

Reppel, Michael ^{[9
]}

Hescheler, Juergen ^{[9
]}

Sievert, Karl-Dietrich ^{[3
]}

Stenzl, Arnulf ^{[3
]}

Skutella, Thomas ^{[1
,6
]}

机构：

[1] Inst Anat, Dept Expt Embryol, D-72074 Tubingen, Germany

[2] Inst Anat, Dept Cellular Neurobiol, D-72074 Tubingen, Germany

[3] Univ Clin Tubingen, Dept Urol, D-72076 Tubingen, Germany

[4] Univ Clin Tubingen, Inst Anthropol & Human Genet, Microarray Facil, D-72076 Tubingen, Germany

[5] Univ Clin Tubingen, ZMF Res Labs, D-72072 Tubingen, Germany

[6] Ctr Regenerat Biol & Med ZRM, D-72076 Tubingen, Germany

[7] Univ Clin Tubingen, Dept Internal Med 2, D-72076 Tubingen, Germany

[8] Kings Coll London, Stem Cell Biol Lab, Wolfson Ctr Age Related Dis, London SE1 1UL, England

[9] Univ Cologne, Inst Neurophysiol, D-50931 Cologne, Germany

来源：

NATURE | 2008年 / 456卷 / 7220期

关键词：

D O I：

10.1038/nature07404

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Human primordial germ cells and mouse neonatal and adult germline stem cells are pluripotent and show similar properties to embryonic stem cells. Here we report the successful establishment of human adult germline stem cells derived from spermatogonial cells of adult human testis. Cellular and molecular characterization of these cells revealed many similarities to human embryonic stem cells, and the germline stem cells produced teratomas after transplantation into immunodeficient mice. The human adult germline stem cells differentiated into various types of somatic cells of all three germ layers when grown under conditions used to induce the differentiation of human embryonic stem cells. We conclude that the generation of human adult germline stem cells from testicular biopsies may provide simple and non- controversial access to individual cell- based therapy without the ethical and immunological problems associated with human embryonic stem cells.

引用

页码：344 / U25

页数：8

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