Skeletal muscle L-type Ca2+ current modulation in γ1-deficient and wildtype murine myotubes by the γ1 subunit and cAMP

Modulation of the steady-state inactivation and current amplitude by the gamma1 subunit of the murine skeletal muscle L-type Ca2+ channel were investigated using the whole-cell patch-clamp technique. Transient expression of the gamma1 subunit, but not of the gamma2 (stargazin) protein, in primary cultured myotubes from gamma1-deficient mice shifted the steady-state inactivation approximately -15 mV, thereby restoring wildtype (WT) steady-state inactivation and current amplitude. The increased Ca2+ current amplitude in gamma1-deficient cells was abolished in myotubes from animals of 4 weeks and older whereas the positive shift in steady-state inactivation was independent of mouse age. Raising intracellular cAMP levels using the membrane-permeant analogue 8-Br-cAMP led to an increase in Ca2+ current amplitude in WT cells to the level in gamma1-deficient myotubes. There was no effect on the current amplitude in gamma1-deficient cells or on the steady-state inactivation in either genotype. Rp-cAMPS, a competitive inhibitor of cAMP-dependent protein kinase, had no effect on the WT Ca2+ current amplitude and steady-state inactivation, but diminished the current amplitude in gamma1-deficient myotubes without affecting the steady-state inactivation in these cells. These data show that the increased Ca2+ influx in myotubes lacking the gamma1 subunit, due to right-shifted steady-state inactivation and increased L-type Ca2+ current amplitude, is determined by the gamma1 subunit. The effect on current amplitude depends on the age of the mice and its cAMP-dependent modulation appears to be controlled by they gamma1 subunit.