Deoxyribonucleotide-containing RNAs: a novel class of templates for HIV-1 reverse transcriptase

被引：15

作者：

Gudima, SO ^{[1
]}

Kazantseva, EG ^{[1
]}

Kostyuk, DA ^{[1
]}

Shchaveleva, IL ^{[1
]}

Grishchenko, OI ^{[1
]}

Memelova, LV ^{[1
]}

Kochetkov, SN ^{[1
]}

机构：

[1] VA ENGELHARDT MOL BIOL INST,MOSCOW 117984,RUSSIA

来源：

NUCLEIC ACIDS RESEARCH | 1997年 / 25卷 / 22期

关键词：

D O I：

10.1093/nar/25.22.4614

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Deoxyribonucleotide-containing RNA-like polynucleotides (dcRNAs) were synthesized by mutant T7 RNA polymerase and their structures confirmed by sequencing. dcRNAs annealed with a 20mer oligodeoxyribonucleotide primer were tested as templates/primers in the reverse transcription reaction catalyzed by HIV-1 reverse transcriptase (RT). All dcRNAs were shown to be efficient templates for both wild-type RT and RT mutants, containing 'AZT-resistant' mutations. Differences in the patterns of the DNA products of RNA-and dcRNA-driven reverse transcription were demonstrated. The kinetic characteristics for dcRNAs utilization were compared with the corresponding parameters for RNA/DNA and DNA/DNA templates/primers. The respective K-m values for dcRNAs appear to be intermediate between those for RNA and DNA templates. A correlation equation connecting apparent K-m value for template/primer and the number of deoxyribonucleotide substitutions in RNA template is proposed.

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页码：4614 / 4618

页数：5

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