Synthesis and properties of oligonucleotide duplexes containing donor and acceptor fluorophores at 2′-positions

被引：18

作者：

Yamana, K ^{[1
]}

Mitsui, T ^{[1
]}

Nakano, H ^{[1
]}

机构：

[1] Himeji Inst Technol, Dept Appl Chem, Himeji, Hyogo 6712201, Japan

来源：

TETRAHEDRON | 1999年 / 55卷 / 30期

关键词：

D O I：

10.1016/S0040-4020(99)00484-6

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A method for introduction of dimethylamionapthamide and fluorescein labels as a fluorescence energy donor and acceptor pair into 2'-positions of DNA duplexes has been described. It has been shown that the attachment of these bulky fluorophores to the sugar 2'-position at the terminal fraying end of each oligonucleotide strand does not alter the normal thermal stability and global conformation of the DNA duplexes. A clear dependence of fluorescence energy transfer efficiency on the number of nucleotides in DNA has been observed, suggesting that the present donor-acceptor pair may be useful for FRET indicator of DNA. (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：9143 / 9150

页数：8

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