Identification of nitrated proteins in Alzheimer's disease brain using a redox proteomics approach

被引：303

作者：

Sultana, R

Poon, HF

Cai, J

Pierce, WM

Merchant, M

Klein, JB

Markesbery, WR

Butterfield, DA ^{[1
]}

机构：

[1] Univ Kentucky, Ctr Membrane Sci, Dept Chem, Lexington, KY 40506 USA

[2] Univ Kentucky, Sanders Brown Ctr Aging, Lexington, KY 40506 USA

[3] Univ Louisville, Sch Med, Dept Pharmacol, Louisville, KY 40202 USA

[4] VAMC, Louisville, KY 40202 USA

[5] Univ Louisville, Core Proteom Lab, Louisville, KY 40208 USA

[6] Univ Kentucky, Dept Neurol & Pathol, Lexington, KY 40536 USA

来源：

NEUROBIOLOGY OF DISEASE | 2006年 / 22卷 / 01期

关键词：

Alzheimer's disease; hippocampus; ATP synthase alpha chain; voltage-dependent anion-selective channel protein I; carbonic anhydrase II; alpha enolase; glyceraldehyde-3-phosphate dehydrogenase; redox proteomics;

D O I：

10.1016/j.nbd.2005.10.004

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Nitric oxide (NO) has been implicated in the pathophysiology of a number of neurodegenerative diseases including Alzheimer's disease (AD). In the present study, using a proteomics approach, we identified enolase, glyceraldehyde-3-phosphate dehydrogenase, ATP synthase alpha chain, carbonic anhydrase-II, and voltage-dependent anion channel-protein as the targets of nitration in AD hippocampus, a region that shows a extensive deposition of amyloid beta-peptide, compared with the age-matched control brains. Immunoprecipitation and Western blotting techniques were used to validate the correct identification of these proteins. Our results are discussed in context of the role of oxidative stress as one of the important mechanisms of neurodegeneration in AD. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：76 / 87

页数：12

共 94 条

[1] The expression of several mitochondrial and nuclear genes encoding the subunits of electron transport chain enzyme complexes, cytochrome c oxidase, and NADH dehydrogenase, in different brain regions in Alzheimer's disease [J].