Selection of streptavidin binders firom a DNA-encoded chemical library

被引：54

作者：

Dumelin, CE ^{[1
]}

Scheuermann, J ^{[1
]}

Melkko, S ^{[1
]}

Neri, D ^{[1
]}

机构：

[1] Swiss Fed Inst Technol, Dept Chem & Appl Biosci, Inst Pharmaceut Sci, CH-8093 Zurich, Switzerland

来源：

BIOCONJUGATE CHEMISTRY | 2006年 / 17卷 / 02期

关键词：

D O I：

10.1021/bc050282y

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA-encoded libraries of small organic molecules facilitate the construction of large, encoded self-assembling chemical libraries for the identification of high-affinity binders to protein targets. We have constructed a library of 477 chemical compounds, coupled to 48mer-oligonucleotides, each containing a unique six-base sequence serving as "bar-code" for the identification of the chemical moiety. The functionality of the library was confirmed by selection and amplification of both high- and low-affinity binding molecules specific to streptavidin.

引用

页码：366 / 370

页数：5

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